Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis | Aloe arborescens |
Protein Variants | Comment | Organism |
---|---|---|
F80A/Y82A/M207G | site-directed mutagenesis, the total cavity volume of the F80A/Y82A/M207G triple mutant is 4fold larger than that of the wild-type pentaketide chromone synthase. The mutant not only catalyzes the iterative condensation of nine molecules of malonyl-CoA, to produce anovel nonaketide naphthopyrone, but also alters the mechanism of the cyclization to produce the angular naphthopyrone with a fused tricyclic ring system | Aloe arborescens |
M207G | site-directed mutagenesis, the pentaketide chromone synthase M207G mutant no longer produces the pentaketide chromone but instead efficiently catalyzes sequential condensations of eight molecules of malonyl-CoA to produce a 1:4 mixture of the octaketides SEK4/SEK4b. The pentaketide-producing pentaketide chromone synthaseis thus transformed into an octaketide synthase by the single-amino acid replacement | Aloe arborescens |
additional information | oktaketide synthase, EC 2.3.1.-, and pentaketide chromone synthase, EC 2.3.1.216, are not functionally interconvertible by the single amino acid switch at residue 207 | Aloe arborescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, about, sequence calculation | Aloe arborescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5 malonyl-CoA | Aloe arborescens | - |
5 CoA + 5,7-dihydroxy-2-methyl-4H-chromen-4-one + 5 CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aloe arborescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5 malonyl-CoA | - |
Aloe arborescens | 5 CoA + 5,7-dihydroxy-2-methyl-4H-chromen-4-one + 5 CO2 + H2O | - |
? | |
additional information | recombinant pentaketide chromone synthasedoes not produce chalcone from 4-coumaroyl-CoA, but instead efficiently accepts malonyl-CoA as a sole substrate to carry out iterative condensations of five molecules of malonyl-CoA, producing a pentaketide, 5,7-dihydroxy-2-methylchromone. Recombinant pentaketide chromone synthaseaccepts acetyl-CoA, resulting from decarboxylation of malonyl-CoA, as a starter substrate | Aloe arborescens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, about, sequence calculation | Aloe arborescens |
Synonyms | Comment | Organism |
---|---|---|
PCS | - |
Aloe arborescens |
pentaketide chromone synthase | - |
Aloe arborescens |
General Information | Comment | Organism |
---|---|---|
evolution | pentaketide chromone synthase is a plant-specific type III polyketide synthase that belongs to the chalcone synthase superfamily of type III polyketide synthases and grouped with other non-chalcone forming enzymes | Aloe arborescens |
additional information | the critical active-site residue 197, and the catalytic triad, Cys164, His303, and Asn336, are conserved in type III polyketide synthases, while the residues lining the active-site are exchanged to M197, L256, V338 (numbering in Madia sativa CHS). Active-site architecture of pentaketide chromone synthase compared to other type III polyketide synthases, crystal structure and hmology modeling, overview | Aloe arborescens |