Protein Variants | Comment | Organism |
---|---|---|
I214L/L215F | site-directed mutagenesis, the mutation restores the active site residues of chalcone synthase, the mutant shows chalcone-forming activity, EC 2.3.1.74. The mutant enzyme thus exhibits 36fold decreases in kcat/Km for 4-coumaroyl-CoA and 20fold decreases in kcat/Km for malonyl-CoA compared with wild-type BAS, kinetics of chalcone naringenin-forming activity at pH 6.5, overview | Rheum palmatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
4-coumaroyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum | |
0.0233 | - |
malonyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | Rheum palmatum | - |
2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rheum palmatum | Q94FV7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | - |
Rheum palmatum | 2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional homology modeling, structure-function relationship , overview | Rheum palmatum |
Synonyms | Comment | Organism |
---|---|---|
bAS | - |
Rheum palmatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rheum palmatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0296 | - |
malonyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum | |
0.0298 | - |
4-coumaroyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rheum palmatum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily. BAS lacks the critical residue Phe215, that is important in the polyketide formation reactions, structure-function relationship of the plant type III PKSs, overview. The absence of Phe215 in BAS accounts for the interruption of the polyketide chain elongation at the diketide stage | Rheum palmatum |
additional information | the absence of Phe215 in BAS accounts for the interruption of the polyketide chain elongation at the diketide stage compared to chalcone synthase, EC 2.3.1.74, structure-function relationship , overview. BAS catalytic residues are Asn336, His303, and Cys164, active site structure, overview | Rheum palmatum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.27 | - |
malonyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum | |
2.96 | - |
4-coumaroyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum |