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Literature summary for 2.3.1.212 extracted from

  • Abe, I.; Sano, Y.; Takahashi, Y.; Noguchi, H.
    Site-directed mutagenesis of benzalacetone synthase. The role of the Phe215 in plant type III polyketide synthases (2003), J. Biol. Chem., 278, 25218-25226.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
I214L/L215F site-directed mutagenesis, the mutation restores the active site residues of chalcone synthase, the mutant shows chalcone-forming activity, EC 2.3.1.74. The mutant enzyme thus exhibits 36fold decreases in kcat/Km for 4-coumaroyl-CoA and 20fold decreases in kcat/Km for malonyl-CoA compared with wild-type BAS, kinetics of chalcone naringenin-forming activity at pH 6.5, overview Rheum palmatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
4-coumaroyl-CoA pH 8.0, 30°C, recombinant wild-type enzyme Rheum palmatum
0.0233
-
malonyl-CoA pH 8.0, 30°C, recombinant wild-type enzyme Rheum palmatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaroyl-CoA + malonyl-CoA + H2O Rheum palmatum
-
2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
?

Organism

Organism UniProt Comment Textmining
Rheum palmatum Q94FV7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + malonyl-CoA + H2O
-
Rheum palmatum 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
?

Subunits

Subunits Comment Organism
More three-dimensional homology modeling, structure-function relationship , overview Rheum palmatum

Synonyms

Synonyms Comment Organism
bAS
-
Rheum palmatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rheum palmatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0296
-
malonyl-CoA pH 8.0, 30°C, recombinant wild-type enzyme Rheum palmatum
0.0298
-
4-coumaroyl-CoA pH 8.0, 30°C, recombinant wild-type enzyme Rheum palmatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Rheum palmatum

General Information

General Information Comment Organism
evolution the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily. BAS lacks the critical residue Phe215, that is important in the polyketide formation reactions, structure-function relationship of the plant type III PKSs, overview. The absence of Phe215 in BAS accounts for the interruption of the polyketide chain elongation at the diketide stage Rheum palmatum
additional information the absence of Phe215 in BAS accounts for the interruption of the polyketide chain elongation at the diketide stage compared to chalcone synthase, EC 2.3.1.74, structure-function relationship , overview. BAS catalytic residues are Asn336, His303, and Cys164, active site structure, overview Rheum palmatum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.27
-
malonyl-CoA pH 8.0, 30°C, recombinant wild-type enzyme Rheum palmatum
2.96
-
4-coumaroyl-CoA pH 8.0, 30°C, recombinant wild-type enzyme Rheum palmatum