Crystallization (Comment) | Organism |
---|---|
purified enzyme, sitting drop vapour diffusion method, mixing of 500 nl of protein solution containing 20 mg/ml protein in 20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, and 2 mM DTT, with 500 nl of reservoir solution containing 100 mM MES-NaOH, pH 6.5, 15% w/v PEG 8000, and 200 mM potassium thiocyanate, and equilibration against 0.1 ml of reservoir solution, 3 days, cryoprotection by 20% v/v glycerol in reservoir solution, X-ray diffraction structure determination and analysis at 1.8 A resolution | Rheum palmatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | Rheum palmatum |
83000 | - |
gel filtration | Rheum palmatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | Rheum palmatum | - |
2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rheum palmatum | Q94FV7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | - |
Rheum palmatum | 2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? | |
4-coumaroyl-CoA + malonyl-CoA + H2O | one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the diketide 4-(4-hydroxyphenyl)-but-3-en-2-one | Rheum palmatum | 2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 43000, SDS-PAGE | Rheum palmatum |
More | structure-function relationship, overview | Rheum palmatum |
Synonyms | Comment | Organism |
---|---|---|
bAS | - |
Rheum palmatum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily | Rheum palmatum |
additional information | structure-function relationship, overview | Rheum palmatum |