Literature summary for 2.3.1.211 extracted from

Kim, E.J.; Cha, M.N.; Kim, B.G.; Ahn, J.H.
Production of curcuminoids in engineered Escherichia coli (2017), J. Microbiol. Biotechnol., 27, 975-982 .

Search for more literature for 2.3.1.211

Application

Application	Comment	Organism
synthesis	production of curcuminoids in engineered Escherichia coli. PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS (curcumin synthase) genes, are introduced into Escherichia coli, and each strain produces dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids in Escherichia coli, the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, is engineered. Using the engineered strains, the production of bisdemethoxycurcumin increases from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l	Oryza sativa Japonica Group
synthesis	production of curcuminoids in engineered Escherichia coli. Two curcuminoids (dicinnamoylmethane and bisdemethoxycurcumin) are synthesized from glucose in Escherichia coli. PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS genes, are introduced into Escherichia coli, and each strain produces dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids in Escherichia coli, the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, is engineered. Using the engineered strains, the production of bisdemethoxycurcumin increases from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l	Oryza sativa Japonica Group

Cloned(Commentary)

Cloned (Comment)	Organism
PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS genes, are introduced into Escherichia coli	Oryza sativa Japonica Group

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa Japonica Group	Q8LIL0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 4-coumaroyl-CoA + malonyl-CoA + H2O	-	Oryza sativa Japonica Group	3 CoA + bisdemethoxycurcumin + 2 CO2	-	?

Synonyms

Synonyms	Comment	Organism
curcumin synthase	-	Oryza sativa Japonica Group
CUS	-	Oryza sativa Japonica Group
OsCUS	-	Oryza sativa Japonica Group

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)