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Literature summary for 2.3.1.211 extracted from

  • Wang, S.; Zhang, S.; Zhou, T.; Zeng, J.; Zhan, J.
    Design and application of an in vivo reporter assay for phenylalanine ammonia-lyase (2013), Appl. Microbiol. Biotechnol., 97, 7877-7885.
    View publication on PubMed

Application

Application Comment Organism
analysis engineering of an in vivo reporter assay for phenylalanine ammonia-lyase efficiency in Escherichia coli based on a plant type III polyketide biosynthetic pathway. The candidate phenylalanine ammonia-lyases are coexpressed with 4-coumarate:CoA ligase 4CL1 from Arabidopsis thaliana and curcuminoid synthase from Oryza sativa. A microplate-based assay is used to measure the titer of dicinnamoylmethane Oryza sativa
synthesis an engineered Escherichia coli strain expressing phenylalanine ammonia-lyase, 4-coumarate:CoA ligase 4CL1 from Arabidopsis thaliana and curcuminoid synthase from Oryza sativa leads to the production of dicinnamoylmethane at a high level of 0.36 g/l. Supplement of 2-fluoro-phenylalanine yields fluorinated dicinnamoylmethane derivatives, 6,6'-difluorodicinnamoylmethane and 6-fluoro-dicinnamoylmethane, of which the latter is a new curcuminoid Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q8LIL0 subsp. japonica
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Synonyms

Synonyms Comment Organism
Os07g0271500
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Oryza sativa