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Literature summary for 2.3.1.211 extracted from

  • Katsuyama, Y.; Matsuzawa, M.; Funa, N.; Horinouchi, S.
    Production of curcuminoids by Escherichia coli carrying an artificial biosynthesis pathway (2008), Microbiology, 154, 2620-2628.
    View publication on PubMed
Search for more literature for 2.3.1.211

Protein Variants

Protein Variants Comment Organism
additional information construction of an artificial curcuminoid biosynthetic pathway, including reactions of phenylalanine ammonia-lyase from the yeast Rhodotorula rubra, 4-coumarate:CoA ligase from Lithospermum erythrorhizon and curcuminoid synthase from Oryza sativa in Escherichia coli for the production of curcuminoids. Cultivation of the recombinant Escherichia coli cells in the presence of tyrosine or phenylalanine, or both, leads to production of bisdemethoxycurcumin, dicinnamoylmethane and cinnamoyl-4-coumaroylmethane, overview. Another Escherichia coli system carrying the 4-coumarate:CoA ligase and the CUS genes is also useful for high-yield production of curcuminoids from exogenously supplemented phenylpropanoid acids 4-coumaric acid, cinnamic acid and ferulic acid Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 4-coumaroyl-CoA + malonyl-CoA + H2O Oryza sativa
-
 3 CoA + bisdemethoxycurcumin + 2 CO2
-
 ?
2 cinnamoyl-CoA + malonyl-CoA + H2O Oryza sativa
-
 3 CoA + dicinnamoylmethane + 2 CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 4-coumaroyl-CoA + malonyl-CoA + H2O = 3 CoA + bisdemethoxycurcumin + 2 CO2 the reaction proceeds in three steps via formation of 4-coumaroyldiketide-CoA and 4-coumaroyl beta-keto acid, overview Oryza sativa
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 4-coumaroyl-CoA + malonyl-CoA + H2O
-
 Oryza sativa 3 CoA + bisdemethoxycurcumin + 2 CO2
-
 ?
2 cinnamoyl-CoA + malonyl-CoA + H2O
-
 Oryza sativa 3 CoA + dicinnamoylmethane + 2 CO2
-
 ?
4-coumaroyl-CoA + cinnamoyl-CoA + malonyl-CoA + H2O
-
 Oryza sativa 3 CoA + cinnamoyl-4-coumaroyl-methane + 2 CO2
-
 ?

Synonyms

Synonyms Comment Organism
curcuminoid synthase
-
 Oryza sativa
CUS
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
 in vivo assay at Oryza sativa