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Literature summary for 2.3.1.21 extracted from

  • Price, N.T.; Jackson, V.N.; van der Leij, F.R.; Cameron, J.M.; Travers, M.T.; Bartelds, B.; Huijkman, N.C.; Zammit, V.A.
    Cloning and expression of the liver and muscle isoforms of ovine carnitine palmitoyltransferase 1: residues within the N-terminus of the muscle isoform influence the kinetic properties of the enzyme (2003), Biochem. J., 372, 871-879.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Ovis aries

Protein Variants

Protein Variants Comment Organism
additional information chimaera in which the distinctive N-terminal segmant of ovine m-BPT 1 is replaced with that from rat M-CPT 1. The ovine N-terminal segment influences the kinetics of the enzyme for both its substrates, such that the Km for palmitoyl-CoA is decreased and that for carnitine is increased for the chimera, relative to the parental enzyme M-CPT 1 from Ovis aries Ovis aries

Inhibitors

Inhibitors Comment Organism Structure
malonyl-CoA IC50 for wild-type L-CPT-1 is 0.0298 mM; IC50 for wild-type M-CPT-1 is 0.010 mM Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
palmitoyl-CoA chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1 Ovis aries
0.0374
-
palmitoyl-CoA wild-type L-CPT1 Ovis aries
0.0676
-
palmitoyl-CoA wild-type M-CPT1 Ovis aries
0.215
-
L-carnitine wild-type L-CPT 1 Ovis aries
0.56
-
L-carnitine wild-type M-CPT 1 Ovis aries
0.785
-
L-carnitine chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1 Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries Q7YQR7
-
-
Ovis aries Q8SPI7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
Ovis aries
-
brain
-
Ovis aries
-
brain high level of mRNA Ovis aries
-
heart
-
Ovis aries
-
heart high level of mRNA Ovis aries
-
kidney
-
Ovis aries
-
kidney high level of mRNA Ovis aries
-
liver
-
Ovis aries
-
liver high level of mRNA Ovis aries
-
lung
-
Ovis aries
-
mammary gland lactating, high level of mRNA Ovis aries
-
mammary gland non-lactating, high level of mRNA Ovis aries
-
additional information no activity in spleen Ovis aries
-
pancreas
-
Ovis aries
-
skeletal muscle
-
Ovis aries
-
skeletal muscle high level of mRNA Ovis aries
-
spleen
-
Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + L-carnitine
-
Ovis aries CoA + L-palmitoylcarnitine
-
?

Synonyms

Synonyms Comment Organism
CPT1A
-
Ovis aries
CPT1B
-
Ovis aries
L-CPT 1
-
Ovis aries
M-CPT 1
-
Ovis aries

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
IC50 for wild-type M-CPT-1 is 0.010 mM Ovis aries malonyl-CoA
0.0298
-
IC50 for wild-type L-CPT-1 is 0.0298 mM Ovis aries malonyl-CoA