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Literature summary for 2.3.1.21 extracted from

  • Nicot, C.; Hegardt, F.G.; Woldegiorgis, G.; Haro, D.; Marrero, P.F.
    Pig liver carnitine palmitoyltransferase I, with low Km for carnitine and high sensitivity to malonyl-CoA inhibition, is a natural chimera of rat liver and muscle enzymes (2001), Biochemistry, 40, 2260-2266.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
2 isoforms of CPT I, liver L-CPT I, skeletal muscle M-CPT I, cloned from liver, expression in Pichia pastoris GS115, DNA sequence determination Sus scrofa
in vitro transcription and translation of L-CPT I Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
malonyl-CoA L-CPT I Sus scrofa
additional information L-CPT I behaves like a natural chimera of M-CPT I and L-CPT I due to malonyl-CoA sensitivity and Km values compared to the enzymes of other organisms Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information L-CPT I behaves like a natural chimera of M-CPT I and L-CPT I due to malonyl-CoA sensitivity and Km values compared to the enzymes of other organisms Sus scrofa
0.035
-
palmitoyl-CoA recombinant wild-type L-CPT I Sus scrofa
0.126
-
L-carnitine recombinant wild-type L-CPT I Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial outer membrane CPT I is located in the outer membrane Sus scrofa 5741
-
mitochondrion 2 isoforms of CPT I, L-CPT I and M-CPT I Sus scrofa 5739
-
mitochondrion recombinant L-CPT I and M-CPT I in Pichia pastoris Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
x * 82000, L-CPT I, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + L-carnitine Sus scrofa
-
CoA + L-acylcarnitine
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
2 isoforms of CPT I in liver mitochondria, liver L-CPT I, skeletal muscle M-CPT I
-

Source Tissue

Source Tissue Comment Organism Textmining
duodenum L-CPT I Sus scrofa
-
heart L-CPT I Sus scrofa
-
heart M-CPT I Sus scrofa
-
kidney L-CPT I Sus scrofa
-
liver L-CPT I Sus scrofa
-
liver M-CPT I Sus scrofa
-
liver CPT I Sus scrofa
-
skeletal muscle M-CPT I Sus scrofa
-
testis L-CPT I, not M-CPT I Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + L-carnitine
-
Sus scrofa CoA + L-acylcarnitine
-
?
palmitoyl-CoA + L-carnitine
-
Sus scrofa CoA + L-palmitoylcarnitine
-
?

Subunits

Subunits Comment Organism
? x * 82000, L-CPT I, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Sus scrofa