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Literature summary for 2.3.1.21 extracted from

  • Johnson, T.M.; Mann, W.R.; Dragland, C.J.; Anderson, R.C.; Nemecek, G.M.; Bell, P.A.
    Over-expression and characterization of active recombinant rat liver carnitine palmitoyltransferase II using baculovirus (1995), Biochem. J., 309, 689-693.
No PubMed abstract available
Search for more literature for 2.3.1.21

Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression of CPT II in Escherichia coli, overexpression of CPT II in Spodoptera frugiperda Sf 9 cells via baculavirus infection, DNA sequence analysis Rattus norvegicus

General Stability

General Stability Organism
no loss in activity after 6 freeze/thaw cycles at -70°C or room temeprature, recombinant CPT II Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 palmitoyl-CoA recombinant CPT II Rattus norvegicus
0.95
-
 L-carnitine recombinant CPT II Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + L-carnitine Rattus norvegicus
-
 CoA + L-acylcarnitine
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 enzyme form CPT II
-

Purification (Commentary)

Purification (Comment) Organism
recombinant CPT II from Sf 9 insect cells Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver CPT II Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.43
-
 recombinant CPT II from Sf 9 insect cells Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, pH 7.0-10.0, 24 h stable, recombinant CPT II Rattus norvegicus
room temperature, 6.5 h, no loss in activity, recombinant CPT II Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-acylcarnitine
-
 ?
palmitoyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-palmitoylcarnitine
-
 ?

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 stable for 24 h at 4°C, recombinant CPT II Rattus norvegicus