Cloned (Comment) | Organism |
---|---|
gene pks, cDNA and amino acid sequence determination and analysis, overexpression of His-tagged PKS in Escherichia coli strain M15 | Cannabis sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42700 | - |
x * 42700, about, sequence calculation, x * 45000, SDS-PAGE | Cannabis sativa |
45000 | - |
x * 42700, about, sequence calculation, x * 45000, SDS-PAGE | Cannabis sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cannabis sativa | - |
gene pks | - |
Cannabis sativa Four-way | - |
gene pks | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Cannabis sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + hexanoyl-CoA | formation of a C12-polyketide, which is formed to olivetol and its carboxylated derivative olivetolic acid by an aldol reaction thrugh stilbene carboxylate synthase-like activity, overview | Cannabis sativa | ? | - |
? | |
3 malonyl-CoA + hexanoyl-CoA | formation of a C12-polyketide, which is formed to olivetol and its carboxylated derivative olivetolic acid by an aldol reaction thrugh stilbene carboxylate synthase-like activity, overview | Cannabis sativa Four-way | ? | - |
? | |
additional information | usage of 4-coumaroyl-CoA and malonyl-CoA as substrates, resulting product is naringenin, not resveratrol. The enzyme might show a broad specificity of substrate including aliphatic substrates such as isovaleryl-CoA, isobutyryl-CoA, and n-hexanoyl-CoA | Cannabis sativa | ? | - |
? | |
additional information | usage of 4-coumaroyl-CoA and malonyl-CoA as substrates, resulting product is naringenin, not resveratrol. The enzyme might show a broad specificity of substrate including aliphatic substrates such as isovaleryl-CoA, isobutyryl-CoA, and n-hexanoyl-CoA | Cannabis sativa Four-way | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42700, about, sequence calculation, x * 45000, SDS-PAGE | Cannabis sativa |
Synonyms | Comment | Organism |
---|---|---|
polyketide synthase | - |
Cannabis sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cannabis sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Cannabis sativa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cannabis sativa | sequence calculation | - |
6.04 |
General Information | Comment | Organism |
---|---|---|
physiological function | polyketide synthases play an important role in the biosynthesis of secondary metabolites such as resveratrol, a candidate for cancer chemoprevention and naringenin, the precursor for flavonoids. Olivetolic acid is also be expected to be synthesized by a PKS | Cannabis sativa |