Cloned (Comment) | Organism |
---|---|
gene AFUA_2G18020, DNA and amino acid seqquence determmination and analysis, sequence comparison, expression of His6-tagged FgaAT in Escherichia coli | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the FgaAT reaction apparently follows Michaelis-Menten kinetics | Aspergillus fumigatus | |
0.122 | - |
acetyl-CoA | pH not specified in the publication, 37°C, recombinant His6-tagged enzyme | Aspergillus fumigatus | |
0.147 | - |
fumigaclavine B | pH not specified in the publication, 37°C, recombinant His6-tagged enzyme | Aspergillus fumigatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 22% activation at 5 mM | Aspergillus fumigatus | |
additional information | no effects by Na+ and K+ at 5 mM, the acetyl-transfer reaction catalysed by FgaAT is very likely independent of the presence of metal ions | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE | Aspergillus fumigatus |
56700 | - |
1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE | Aspergillus fumigatus |
57000 | - |
1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE | Aspergillus fumigatus |
60800 | - |
recombinant His6-tagged enzyme, gel filtration | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + fumigaclavine B | Aspergillus fumigatus | - |
CoA + fumigaclavine A | - |
? | |
acetyl-CoA + fumigaclavine B | Aspergillus fumigatus Af293 | - |
CoA + fumigaclavine A | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4WZ64 | gene AFUA_2G18020 | - |
Aspergillus fumigatus Af293 | Q4WZ64 | gene AFUA_2G18020 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + fumigaclavine B | - |
Aspergillus fumigatus | CoA + fumigaclavine A | - |
? | |
acetyl-CoA + fumigaclavine B | - |
Aspergillus fumigatus Af293 | CoA + fumigaclavine A | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
FgaAT | - |
Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
acetyl-CoA | pH not specified in the publication, 37°C, recombinant His6-tagged enzyme | Aspergillus fumigatus | |
0.6 | - |
fumigaclavine B | pH not specified in the publication, 37°C, recombinant His6-tagged enzyme | Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
physiological function | FgaAT catalyses the acetylation of fumigaclavine B, the conversion of fumigaclavine B to A, which belong to the ergot alkaloids of the clavine-type | Aspergillus fumigatus |