BRENDA - Enzyme Database
show all sequences of 2.3.1.203

Biochemical analysis and structure determination of bacterial acetyltransferases responsible for the biosynthesis of UDP-N,N-diacetylbacillosamine

Morrison, M.J.; Imperiali, B.; J. Biol. Chem. 288, 32248-32260 (2013)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
apo-crystal structures of the acetyltransferase domain ATD and in complex with AcCoA. The N-terminal section (Asn199-Leu285) comprises a binding pocket for the UDP-4-amino sugar substrate through a beta-alpha-beta-alpha-beta Rossman fold motif. The C-terminus (Pro286-Leu403) is composed of a left-handed beta-helix motif that, in conjunction with an adjacent PglB-ATD protomer in the trimeric state, forms an extended cleft that is utilized for AcCoA binding
Neisseria gonorrhoeae
compared to isoforms PglD and PglB, WeeI contains an additional loop (Gln174-Pro180) that forms the substrate binding pocket near the pyranose moiety. Residue Gln174 seems to be critical for catalysis. When aligned to the PglD UDP-4-amino structure, this position is analogous to Asn162 that interacts with the carbonyl oxygen of the pyranose C2-acetyl group. In the WeeI apo-structure, Gln174 is within 5 A of the catalytic base His138 and 3.6 A of the AcCoA thioester
Acinetobacter baumannii
Engineering
Protein Variants
Commentary
Organism
D332A
active site mutant, strong loss of activity
Neisseria gonorrhoeae
F13A
active site mutant, strong loss of activity
Acinetobacter baumannii
H210F
active site mutant, strong loss of activity
Neisseria gonorrhoeae
Q174A
active site mutant, strong loss of activity
Acinetobacter baumannii
Q369A
active site mutant, strong loss of activity
Neisseria gonorrhoeae
Q370A
active site mutant, 50% loss of activity
Neisseria gonorrhoeae
T176A
active site mutant, strong loss of activity
Acinetobacter baumannii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q370A, pH 7.4, 25°C
Neisseria gonorrhoeae
0.097
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q369A, pH 7.4, 25°C
Neisseria gonorrhoeae
0.099
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Neisseria gonorrhoeae
0.32
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant H210F, pH 7.4, 25°C
Neisseria gonorrhoeae
0.56
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant D332A, pH 7.4, 25°C
Neisseria gonorrhoeae
2.42
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q174A, pH 7.4, 25°C
Acinetobacter baumannii
2.52
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Acinetobacter baumannii
4.51
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant F13A, pH 7.4, 25°C
Acinetobacter baumannii
4.6
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant T176A, pH 7.4, 25°C
Acinetobacter baumannii
Organism
Organism
UniProt
Commentary
Textmining
Acinetobacter baumannii
B0V6J7
-
-
Acinetobacter baumannii AYE
B0V6J7
-
-
Neisseria gonorrhoeae
Q5FAE1
-
-
Neisseria gonorrhoeae FA 1090
Q5FAE1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
-
736434
Neisseria gonorrhoeae
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
-
?
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
-
736434
Acinetobacter baumannii
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
-
?
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
-
736434
Acinetobacter baumannii AYE
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
-
?
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
-
736434
Neisseria gonorrhoeae FA 1090
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ABAYE3807
-
Acinetobacter baumannii
NGO_0085
-
Neisseria gonorrhoeae
WeeI
-
Acinetobacter baumannii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1200
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant H210F, pH 7.4, 25°C
Neisseria gonorrhoeae
1900
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q174A, pH 7.4, 25°C
Acinetobacter baumannii
3000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q370A, pH 7.4, 25°C
Neisseria gonorrhoeae
3600
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant D332A, pH 7.4, 25°C
Neisseria gonorrhoeae
5600
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q369A, pH 7.4, 25°C
Neisseria gonorrhoeae
72000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Neisseria gonorrhoeae
82000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant F13A, pH 7.4, 25°C
Acinetobacter baumannii
330000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant T176A, pH 7.4, 25°C
Acinetobacter baumannii
510000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Acinetobacter baumannii
Crystallization (Commentary) (protein specific)
Crystallization
Organism
apo-crystal structures of the acetyltransferase domain ATD and in complex with AcCoA. The N-terminal section (Asn199-Leu285) comprises a binding pocket for the UDP-4-amino sugar substrate through a beta-alpha-beta-alpha-beta Rossman fold motif. The C-terminus (Pro286-Leu403) is composed of a left-handed beta-helix motif that, in conjunction with an adjacent PglB-ATD protomer in the trimeric state, forms an extended cleft that is utilized for AcCoA binding
Neisseria gonorrhoeae
compared to isoforms PglD and PglB, WeeI contains an additional loop (Gln174-Pro180) that forms the substrate binding pocket near the pyranose moiety. Residue Gln174 seems to be critical for catalysis. When aligned to the PglD UDP-4-amino structure, this position is analogous to Asn162 that interacts with the carbonyl oxygen of the pyranose C2-acetyl group. In the WeeI apo-structure, Gln174 is within 5 A of the catalytic base His138 and 3.6 A of the AcCoA thioester
Acinetobacter baumannii
Engineering (protein specific)
Protein Variants
Commentary
Organism
D332A
active site mutant, strong loss of activity
Neisseria gonorrhoeae
F13A
active site mutant, strong loss of activity
Acinetobacter baumannii
H210F
active site mutant, strong loss of activity
Neisseria gonorrhoeae
Q174A
active site mutant, strong loss of activity
Acinetobacter baumannii
Q369A
active site mutant, strong loss of activity
Neisseria gonorrhoeae
Q370A
active site mutant, 50% loss of activity
Neisseria gonorrhoeae
T176A
active site mutant, strong loss of activity
Acinetobacter baumannii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q370A, pH 7.4, 25°C
Neisseria gonorrhoeae
0.097
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q369A, pH 7.4, 25°C
Neisseria gonorrhoeae
0.099
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Neisseria gonorrhoeae
0.32
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant H210F, pH 7.4, 25°C
Neisseria gonorrhoeae
0.56
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant D332A, pH 7.4, 25°C
Neisseria gonorrhoeae
2.42
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q174A, pH 7.4, 25°C
Acinetobacter baumannii
2.52
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Acinetobacter baumannii
4.51
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant F13A, pH 7.4, 25°C
Acinetobacter baumannii
4.6
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant T176A, pH 7.4, 25°C
Acinetobacter baumannii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
-
736434
Neisseria gonorrhoeae
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
-
?
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
-
736434
Acinetobacter baumannii
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
-
?
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
-
736434
Acinetobacter baumannii AYE
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
-
?
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
-
736434
Neisseria gonorrhoeae FA 1090
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1200
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant H210F, pH 7.4, 25°C
Neisseria gonorrhoeae
1900
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q174A, pH 7.4, 25°C
Acinetobacter baumannii
3000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q370A, pH 7.4, 25°C
Neisseria gonorrhoeae
3600
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant D332A, pH 7.4, 25°C
Neisseria gonorrhoeae
5600
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q369A, pH 7.4, 25°C
Neisseria gonorrhoeae
72000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Neisseria gonorrhoeae
82000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant F13A, pH 7.4, 25°C
Acinetobacter baumannii
330000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant T176A, pH 7.4, 25°C
Acinetobacter baumannii
510000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Acinetobacter baumannii
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
800
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q174A, pH 7.4, 25°C
Acinetobacter baumannii
3700
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant H210F, pH 7.4, 25°C
Neisseria gonorrhoeae
6500
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant D332A, pH 7.4, 25°C
Neisseria gonorrhoeae
18000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant F13A, pH 7.4, 25°C
Acinetobacter baumannii
58000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q369A, pH 7.4, 25°C
Neisseria gonorrhoeae
71000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant T176A, pH 7.4, 25°C
Acinetobacter baumannii
200000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Acinetobacter baumannii
340000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q370A, pH 7.4, 25°C
Neisseria gonorrhoeae
730000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Neisseria gonorrhoeae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
800
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q174A, pH 7.4, 25°C
Acinetobacter baumannii
3700
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant H210F, pH 7.4, 25°C
Neisseria gonorrhoeae
6500
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant D332A, pH 7.4, 25°C
Neisseria gonorrhoeae
18000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant F13A, pH 7.4, 25°C
Acinetobacter baumannii
58000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q369A, pH 7.4, 25°C
Neisseria gonorrhoeae
71000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant T176A, pH 7.4, 25°C
Acinetobacter baumannii
200000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Acinetobacter baumannii
340000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
mutant Q370A, pH 7.4, 25°C
Neisseria gonorrhoeae
730000
-
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose
wild-type, pH 7.4, 25°C
Neisseria gonorrhoeae
Other publictions for EC 2.3.1.203
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736434
Morrison
Biochemical analysis and struc ...
Acinetobacter baumannii, Acinetobacter baumannii AYE, Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090
J. Biol. Chem.
288
32248-32260
2013
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2
7
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9
-
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4
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-
4
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3
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9
-
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-
2
7
-
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9
-
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4
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9
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9
9
718901
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Biochemical characterization o ...
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Biochemistry
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4936-4948
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-
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1
-
-
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-
-
-
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1
-
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1
1
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1
-
-
-
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1
-
-
-
-
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-
-
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720459
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14
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-
-
-
-
-
-
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2
-
1
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1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718849
Rangarajan
Structure and active site resi ...
Campylobacter jejuni
Biochemistry
47
1827-1836
2008
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
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2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714160
Olivier
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Campylobacter jejuni, Campylobacter jejuni NCTC 11168
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45
13659-13669
2006
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1
-
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-
1
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1
-
-
14
-
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1
-
-
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2
1
2
-
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1
-
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1
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1
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1
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1
-
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2
1
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1
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1
1
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1
1