BRENDA - Enzyme Database show
show all sequences of 2.3.1.203

In vitro biosynthesis of UDP-N,N'-diacetylbacillosamine by enzymes of the Campylobacter jejuni general protein glycosylation system

Olivier, N.B.; Chen, M.M.; Behr, J.R.; Imperiali, B.; Biochemistry 45, 13659-13669 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells
Campylobacter jejuni
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
at 37°C, pH 7.7
Campylobacter jejuni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23500
-
x * 23500, His-tagged enzyme, SDS-PAGE
Campylobacter jejuni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Campylobacter jejuni
-
-
-
Campylobacter jejuni NCTC 11168
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA resin column chromatography
Campylobacter jejuni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
-
714160
Campylobacter jejuni
CoA + UDP-N,N'-diacetylbacillosamine
i.e. UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
?
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
-
714160
Campylobacter jejuni NCTC 11168
CoA + UDP-N,N'-diacetylbacillosamine
i.e. UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 23500, His-tagged enzyme, SDS-PAGE
Campylobacter jejuni
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8050
-
UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
at 37°C, pH 7.7
Campylobacter jejuni
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells
Campylobacter jejuni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
at 37°C, pH 7.7
Campylobacter jejuni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23500
-
x * 23500, His-tagged enzyme, SDS-PAGE
Campylobacter jejuni
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA resin column chromatography
Campylobacter jejuni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
-
714160
Campylobacter jejuni
CoA + UDP-N,N'-diacetylbacillosamine
i.e. UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
?
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
-
714160
Campylobacter jejuni NCTC 11168
CoA + UDP-N,N'-diacetylbacillosamine
i.e. UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 23500, His-tagged enzyme, SDS-PAGE
Campylobacter jejuni
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8050
-
UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
at 37°C, pH 7.7
Campylobacter jejuni
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the final step in N,N'-diacetylbacillosamine synthesis by N-acetylation of UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine at the C4 position
Campylobacter jejuni
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the final step in N,N'-diacetylbacillosamine synthesis by N-acetylation of UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine at the C4 position
Campylobacter jejuni
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
20000
-
UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
at 37°C, pH 7.7
Campylobacter jejuni
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
20000
-
UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
at 37°C, pH 7.7
Campylobacter jejuni
Other publictions for EC 2.3.1.203
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736434
Morrison
Biochemical analysis and struc ...
Acinetobacter baumannii, Acinetobacter baumannii AYE, Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090
J. Biol. Chem.
288
32248-32260
2013
-
-
-
2
7
-
-
9
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
2
7
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
718901
Hartley
Biochemical characterization o ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae MS11
Biochemistry
50
4936-4948
2011
-
-
1
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720459
Ding
Identification and quantificat ...
Campylobacter jejuni, Campylobacter jejuni NCTC 11168
Mol. Cell. Proteomics
8
2170-2185
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718849
Rangarajan
Structure and active site resi ...
Campylobacter jejuni
Biochemistry
47
1827-1836
2008
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714160
Olivier
In vitro biosynthesis of UDP-N ...
Campylobacter jejuni, Campylobacter jejuni NCTC 11168
Biochemistry
45
13659-13669
2006
-
-
1
-
-
-
-
1
-
-
1
-
-
14
-
-
1
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
1
1
-
1
1