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Literature summary for 2.3.1.203 extracted from

  • Olivier, N.B.; Chen, M.M.; Behr, J.R.; Imperiali, B.
    In vitro biosynthesis of UDP-N,N'-diacetylbacillosamine by enzymes of the Campylobacter jejuni general protein glycosylation system (2006), Biochemistry, 45, 13659-13669.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
 UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine at 37°C, pH 7.7 Campylobacter jejuni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
 x * 23500, His-tagged enzyme, SDS-PAGE Campylobacter jejuni

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-
Campylobacter jejuni NCTC 11168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
-
 Campylobacter jejuni CoA + UDP-N,N'-diacetylbacillosamine i.e. UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose ?
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine
-
 Campylobacter jejuni NCTC 11168 CoA + UDP-N,N'-diacetylbacillosamine i.e. UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose ?

Subunits

Subunits Comment Organism
? x * 23500, His-tagged enzyme, SDS-PAGE Campylobacter jejuni

Synonyms

Synonyms Comment Organism
Cj1123c
-
 Campylobacter jejuni
PglD
-
 Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8050
-
 UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine at 37°C, pH 7.7 Campylobacter jejuni

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the final step in N,N'-diacetylbacillosamine synthesis by N-acetylation of UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine at the C4 position Campylobacter jejuni

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
20000
-
 UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine at 37°C, pH 7.7 Campylobacter jejuni