Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pLysS cells | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine | at 37°C, pH 7.7 | Campylobacter jejuni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23500 | - |
x * 23500, His-tagged enzyme, SDS-PAGE | Campylobacter jejuni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Campylobacter jejuni NCTC 11168 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine | - |
Campylobacter jejuni | CoA + UDP-N,N'-diacetylbacillosamine | i.e. UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose | ? | |
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine | - |
Campylobacter jejuni NCTC 11168 | CoA + UDP-N,N'-diacetylbacillosamine | i.e. UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23500, His-tagged enzyme, SDS-PAGE | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
Cj1123c | - |
Campylobacter jejuni |
PglD | - |
Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8050 | - |
UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine | at 37°C, pH 7.7 | Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the final step in N,N'-diacetylbacillosamine synthesis by N-acetylation of UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine at the C4 position | Campylobacter jejuni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20000 | - |
UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine | at 37°C, pH 7.7 | Campylobacter jejuni |