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Literature summary for 2.3.1.201 extracted from
- Structural analysis of WbpE from Pseudomonas aeruginosa PAO1: a nucleotide sugar aminotransferase involved in O-antigen assembly (2010), Biochemistry, 49, 7227-7237.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzymes WbpA, WbpB, WbpE, WbpD and WbpI which act stepwise manner starting from UDP-GlcNAc can be combined in vitro to generate UDP-ManNAc(3NAc)A in a single reaction vessel | Pseudomonas aeruginosa PAO1 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas aeruginosa PAO1 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.107 | - |
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | 30°C, pH 7.0 | Pseudomonas aeruginosa PAO1 |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa PAO1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Pseudomonas aeruginosa PAO1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | - |
Pseudomonas aeruginosa PAO1 | CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | - |
? | |
| additional information | no substrate: UDP-GlcNAcA, UDP-GlcNAc, and UDP-2-acetamido-4-amino-2,4,6-trideoxy-D-glucosamine | Pseudomonas aeruginosa PAO1 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pseudomonas aeruginosa PAO1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 65 | - |
Pseudomonas aeruginosa PAO1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas aeruginosa PAO1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | - |
Pseudomonas aeruginosa PAO1 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 48.3 | - |
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | 30°C, pH 7.0 | Pseudomonas aeruginosa PAO1 | |
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Release 2023.1 (January 2023)
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