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Literature summary for 2.3.1.201 extracted from
- Evidence that WbpD is an N-acetyltransferase belonging to the hexapeptide acyltransferase superfamily and an important protein for O-antigen biosynthesis in Pseudomonas aeruginosa PAO1 (2005), Mol. Microbiol., 57, 1288-1303.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as an N-terminally histidine-tagged fusion protein in Escherichia coli BL21(DE3) pLysS cells | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K136A | the mutation reduces the stabilizing effects of acetyl-CoA | Pseudomonas aeruginosa |
| K136R | the mutation showed no discernible effect the stabilizing effects of acetyl-CoA on the purified mutant protein | Pseudomonas aeruginosa |
General Stability
| General Stability | Organism |
|---|---|
| incubation of the enzyme with acetyl-CoA significantly enhances the stability of the protein and prevents precipitation over a course of 14 days | Pseudomonas aeruginosa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22726 | - |
3 * 22726, His-tagged enzyme, calculated from amino acid sequence | Pseudomonas aeruginosa |
| 22726 | - |
3 * 22726, MALDI-TOF mass spectrometry | Pseudomonas aeruginosa |
| 23000 | - |
3 * 23000, His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
| 61000 | - |
His-tagged enzyme, gel filtration | Pseudomonas aeruginosa |
| 68180 | - |
MALDI-TOF mass spectrometry | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
serotype O5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | - |
Pseudomonas aeruginosa | CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 22726, His-tagged enzyme, calculated from amino acid sequence | Pseudomonas aeruginosa |
| homotrimer | 3 * 22726, MALDI-TOF mass spectrometry | Pseudomonas aeruginosa |
| homotrimer | 3 * 23000, His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid3-N-acetyltransferase | - |
Pseudomonas aeruginosa |
| WbpD | - |
Pseudomonas aeruginosa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas aeruginosa | pI-value of above 8.0, isoelectric focusing | - |
8 |
| Pseudomonas aeruginosa | calculated from amino acid sequence | - |
8.2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is required for B-band O-antigen biosynthesis in Pseudomonas aeruginosa | Pseudomonas aeruginosa |
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