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Literature summary for 2.3.1.200 extracted from

  • Christensen, Q.H.; Martin, N.; Mansilla, M.C.; de Mendoza, D.; Cronan, J.E.
    A novel amidotransferase required for lipoic acid cofactor assembly in Bacillus subtilis (2011), Mol. Microbiol., 80, 350-363.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.200

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain QC143 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
C150A the mutation results in loss of enzymatic activity and the inability to form an acyl-enzyme intermediate Bacillus subtilis
C150S the mutation results in loss of enzymatic activity and the inability to form an acyl-enzyme intermediate Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
octanoyl-glycine cleavage system H protein + pyruvate dehydrogenase subunit E2
-
 Bacillus subtilis glycine cleavage system H protein + pyruvate dehydrogenase subunit E2-octanoyl
-
 r

Synonyms

Synonyms Comment Organism
GcvH:E2 amidotransferase
-
 Bacillus subtilis
GcvH:[lipoyl domain] amidotransferase
-
 Bacillus subtilis
LIPL
-
 Bacillus subtilis
ywfL
-
 Bacillus subtilis

General Information

General Information Comment Organism
metabolism the enzyme is essential for lipoic acid synthesis, but has no detectable octanoyltransferase or ligase activity either in vitro or in vivo. The enzyme is required for PdhC modification Bacillus subtilis