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Literature summary for 2.3.1.20 extracted from

  • Rastogi, S.; Singh, A.K.; Chandra, G.; Kushwaha, P.; Pant, G.; Singh, K.; Mitra, K.; Sashidhara, K.V.; Krishnan, M.Y.
    The diacylglycerol acyltransferase Rv3371 of Mycobacterium tuberculosis is required for growth arrest and involved in stress-induced cell wall alterations (2017), Tuberculosis, 104, 8-19 .
    View publication on PubMed
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene Rv3371, specific to pathogenic mycobacteria, leads to modifications in colony morphotype, bacterial architecture, cell surface properties, and to elevated triacylglycerol (TAG) levels when expressed in Mycolicibacterium smegmatis (Mycobacterium smegmatis) Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane mainly Mycobacterium tuberculosis
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol Mycobacterium tuberculosis
-
 CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol Mycobacterium tuberculosis H37Rv
-
 CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol Mycobacterium tuberculosis ATCC 25618
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WKA9
-
-
Mycobacterium tuberculosis ATCC 25618 P9WKA9
-
-
Mycobacterium tuberculosis H37Rv P9WKA9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mycobacterium tuberculosis CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mycobacterium tuberculosis H37Rv CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mycobacterium tuberculosis ATCC 25618 CoA + 1,2,3-triacylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
DGAT
-
 Mycobacterium tuberculosis
diacylglycerol acyltransferase
-
 Mycobacterium tuberculosis
Rv3371
-
 Mycobacterium tuberculosis

Expression

Organism Comment Expression
Mycobacterium tuberculosis the Rv3371 promoter is minimally active during exponential growth in vitro, but is upregulated under stationary phase, hypoxia, nutrient starvation, nitrosative stress, low iron, in IFN-gamma activated macrophages and infected mice. The low iron-induced expression of Rv3371 is likely due to the de-repression by Rv1404, which is probably activated by ideR up

General Information

General Information Comment Organism
malfunction a Rv3371 deletion mutant of Mtb shows impaired non-replicating persistence in vitro and altered sensitivity to anti-mycobacterial drugs. In low iron medium, the Rv3371 deletion mutant shows reduced formation of TAG containing extracellular vesicles Mycobacterium tuberculosis
physiological function the diacylglycerol acyltransferase Rv3371 of Mycobacterium tuberculosis is required for growth arrest and involved in stress-induced cell wall alterations during persistence. It is involved in the biosynthesis of triacylglycerol (TAG). TAG is important to mycobacteria both as cell envelope component and energy reservoir Mycobacterium tuberculosis