Literature summary for 2.3.1.20 extracted from

Tan, W.J.; Yang, Y.C.; Zhou, Y.; Huang, L.P.; Xu, L.; Chen, Q.F.; Yu, L.J.; Xiao, S.
Diacylglycerol acyltransferase and diacylglycerol kinase modulate triacylglycerol and phosphatidic acid production in the plant response to freezing stress (2018), Plant Physiol., 177, 1303-1318 .

Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
gene DGAT1, quantitative RT-pCR expression analysis	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of plant mutant dgat1-1 (CS3861) by methanesulfonate, and of T-DNA insertional mutant dgat1-2 (SALK_039456), phenotypes, comparison of lipid compositions and contents in wild-type and mutant leaves and seeds, detailed overview	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Arabidopsis thaliana	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol	Arabidopsis thaliana	-	CoA + 1,2,3-triacylglycerol	-	?
acyl-CoA + 1,2-diacyl-sn-glycerol	Arabidopsis thaliana Col-0	-	CoA + 1,2,3-triacylglycerol	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9SLD2	-	-
Arabidopsis thaliana Col-0	Q9SLD2	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	temporal expression pattern of DGAT1, overview. DGAT1 expression is upregulated from 3 to 96 h upon cold exposure, with levels increasing 197 and 43fold in the shoots and the roots, respectively, at 96 h	Arabidopsis thaliana	-
root	-	Arabidopsis thaliana	-
rosette leaf	-	Arabidopsis thaliana	-
shoot	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol	-	Arabidopsis thaliana	CoA + 1,2,3-triacylglycerol	-	?
acyl-CoA + 1,2-diacyl-sn-glycerol	-	Arabidopsis thaliana Col-0	CoA + 1,2,3-triacylglycerol	-	?

Synonyms

Synonyms	Comment	Organism
DGAT	-	Arabidopsis thaliana
diacylglycerol acyltransferase	-	Arabidopsis thaliana

Expression

Organism	Comment	Expression
Arabidopsis thaliana	DGAT1 expression is upregulated from 3 to 96 h upon cold exposure, with levels increasing 197 and 43fold in the shoots and the roots, respectively, at 96 h	up

General Information

General Information	Comment	Organism
malfunction	with or without cold acclimation, the dgat1 mutants exhibit higher sensitivity upon freezing exposure compared with the wild-type. Under cold conditions, the dgat1 mutants show reduced expression of C-REPEAT/DRE binding factor 2 and its regulons, which are essential for the acquisition of cold tolerance. Lipid profiling reveals that freezing significantly increases the levels of phosphatidic acid (PA) and diacylglycerol (DAG) while decreasing triacylglycerol (TAG) in the rosettes of dgat1 mutant plants. During freezing stress, the accumulation of PA in dgat1 mutant plants stimulates NADPH oxidase activity and enhances RbohD-dependent hydrogen peroxide production compared with the wild-type. Moreover, the cold-inducible transcripts of DGK2, DGK3, and DGK5, encoding diacylglycerol kinases, are significantly more upregulated in the dgat1 mutants than in the wild-type during cold stress. H2O2 and salicylic acid accumulate in the dgat1 mutants upon exposure to freezing temperatures. The dgat1 mutants show decreased expression of CBF2 and its target genes. Comparisons of lipid compositions and contents in wild-type and mutant leaves and seeds, phenotypes, detailed overview	Arabidopsis thaliana
metabolism	diacylglycerol acyltransferase and diacylglycerol kinase modulate triacylglycerol and phosphatidic acid production in the plant response to freezing stress	Arabidopsis thaliana
physiological function	the conversion of diacylglycerol (DAG) to triacylglycerol (TAG) by DGAT1 is critical for plant freezing tolerance, acting by balancing TAG and phosphatidic acid (PA) production in Arabidopsis thaliana	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)