Cloned (Comment) | Organism |
---|---|
gene DGAT1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli and Saccharomyces cerevisiae double null strain DELZTADGA1/DELTALRO1, recombinant expression of mutant CaDGAT1 in Glycine max | Corylus americana |
gene DGAT1, sequence comparisons, recombinant expression of wild-type and mutant enzymes, recombinant expression in Escherichia coli and Saccharomyces cerevisiae double null strain DELZTADGA1/DELTALRO1 | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a type 1 DGAT complementary DNA (cDNA) from Corylus americana is isolated, CaDGAT1, improved variants from a screened library are created, then the corresponding amino acid substitutions are made in a Glycine max type 1 DGAT. Effects on soybean oil content and composition are determined following expression of the engineered GmDGAT1 in soybean somatic embryos of either the wild-type or the engineered variants of each DGAT, effect on soybean seed composition, detailed overview | Glycine max |
additional information | the type 1 DGAT complementary DNA (cDNA) from Corylus americana is isolated, CaDGAT1, improved variants are created, library screening, and effect on Corylus americana seed composition, detailed overview. The corresponding amino acid substitutions are repeated in a Glycine max type 1 DGAT. Effects on soybean oil content and composition are determined following expression of the engineered GmDGAT1 in soybean somatic embryos of either the wild-type or the engineered variants of each DGAT, analysis of effect on soybean seed composition | Corylus americana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Glycine max | 16020 | - |
membrane | - |
Corylus americana | 16020 | - |
microsome | - |
Glycine max | - |
- |
microsome | - |
Corylus americana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | Glycine max | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
acyl-CoA + 1,2-diacyl-sn-glycerol | Corylus americana | - |
CoA + 1,2,3-triacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corylus americana | A0A161IUT7 | - |
- |
Glycine max | I1MSF2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | developing | Corylus americana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Glycine max | CoA + 1,2,3-triacylglycerol | - |
? | |
acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Corylus americana | CoA + 1,2,3-triacylglycerol | - |
? | |
oleoyl-CoA + 1,2-diacyl-sn-glycerol | assay with radioactive oleoyl-CoA and DAG from microsome preparation of the recombinant yeast cells | Glycine max | CoA + 1,2-diacyl-3-oleoylglycerol | - |
? | |
oleoyl-CoA + 1,2-diacyl-sn-glycerol | assay with radioactive oleoyl-CoA and DAG from microsome preparation of the recombinant yeast cells | Corylus americana | CoA + 1,2-diacyl-3-oleoylglycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61700, about, sequence calculation | Corylus americana |
Synonyms | Comment | Organism |
---|---|---|
CaDGAT1 | - |
Corylus americana |
DGAT1 | - |
Glycine max |
DGAT1 | - |
Corylus americana |
GmDGAT1B | - |
Glycine max |
type 1 diacylglycerol acyltransferase | - |
Glycine max |
type 1 diacylglycerol acyltransferase | - |
Corylus americana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Glycine max |
25 | - |
assay at | Corylus americana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Glycine max |
7.2 | - |
assay at | Corylus americana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Corylus americana | sequence calculation | - |
8.93 |
General Information | Comment | Organism |
---|---|---|
additional information | comparison of Glycine max and Corylus americana DGAT1 sequences | Glycine max |
additional information | comparison of Glycine max and Corylus americana DGAT1 sequences | Corylus americana |