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Literature summary for 2.3.1.20 extracted from

  • Chellamuthu, M.; Kumaresan, K.; Subramanian, S.; Muthumanickam, H.
    Functional analysis of sesame diacylglycerol acyltransferase and phospholipid diacylglycerol acyltransferase genes using in silico and in vitro approaches (2019), Plant Mol. Biol. Rep., 37, 146-156 .
No PubMed abstract available
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene SiDGAT1, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, functional recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiDGAT gene-transformed mutants, lipid analysis, overview Sesamum indicum
gene SiDGAT2, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, functional recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiDGAT gene-transformed mutants, lipid analysis, overview Sesamum indicum

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
 Sesamum indicum 5789
-
lipid droplet
-
 Sesamum indicum 5811
-
soluble SiDGAT1 A1 and SiDGAT1 A2 do not have any transmembrane domains Sesamum indicum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol Sesamum indicum
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Sesamum indicum M1E7W9
-
-
Sesamum indicum XP_011098009
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information tissue distribution analysis by quantitative real-time PCR Sesamum indicum
-
seed highest expression of SiDGAT1 in mature seeds Sesamum indicum
-
seed highest expression of SiDGAT2 in mature seeds Sesamum indicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Sesamum indicum CoA + 1,2,3-triacylglycerol
-
 ?

Subunits

Subunits Comment Organism
? x * 37484, DGAT2, sequence calculation Sesamum indicum
? x * 61936, DGAT1, sequence calculation Sesamum indicum

Synonyms

Synonyms Comment Organism
DGAT1
-
 Sesamum indicum
DGAT2
-
 Sesamum indicum
diacylglycerol acyltransferase
-
 Sesamum indicum
SiDGAT1
-
 Sesamum indicum
SiDGAT2
-
 Sesamum indicum

pI Value

Organism Comment pI Value Maximum pI Value
Sesamum indicum sequence calculation
-
 8.06
Sesamum indicum sequence calculation
-
 9.42

General Information

General Information Comment Organism
metabolism specific role of DGAT (EC 2.3.1.20) and PDAT (EC 2.3.1.158) genes in fatty acid biosynthesis Sesamum indicum
physiological function diacylglycerol acyltransferase (DGAT) is an acyl-CoA-dependent enzyme which converts diacylglycerol (DAG) to triacylglycerol (TAG) Sesamum indicum