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Literature summary for 2.3.1.20 extracted from
- Two clades of type-1 Brassica napus diacylglycerol acyltransferase exhibit differences in acyl-CoA preference (2016), Lipids, 51, 781-786 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene BnaDGAT1.a, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme BnaDGAT1.a in Saccharomyces cerevisiae strain H1246, deficient in TAG synthesis | Brassica napus |
| gene BnaDGAT1.b, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme BnaDGAT1.a in Saccharomyces cerevisiae strain H1246, deficient in TAG synthesis | Brassica napus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | Brassica napus | - |
CoA + 1,2,3-triacylglycerol | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | K9LMJ4 | - |
- |
| Brassica napus | Q9M4V2 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Brassica napus | CoA + 1,2,3-triacylglycerol | - |
? | |
| additional information | although the four forms of recombinant microsomal BnaDGAT1 display enhanced specificity for palmitoyl (16:0)-CoA over oleoyl (18:1DELTA9cis, i.e. 18:1), the enzymes exhibit an enhanced selectivity for 18:1-CoA when assayed with a 3:1 ratio of 18:1-CoA to 16:0-CoA. Substrate specificity and selectivity properties of the recombinant enzymes | Brassica napus | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BnaDGAT1.a | - |
Brassica napus |
| BnaDGAT1.b | - |
Brassica napus |
| DGAT | - |
Brassica napus |
| diacylglycerol acyltransferase | - |
Brassica napus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogeny of these BnaDGAT1 gene forms. Two clades of DGAT1, which appear to have diverged relatively early in Brassicaceae's history, differ in preference for linoleoyl (18:2DELTA9cis12cis, i.e. 18:2-CoA). Phylogenetic analysis | Brassica napus |
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Release 2023.1 (January 2023)
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