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Literature summary for 2.3.1.20 extracted from

  • Gajdos, P.; Ledesma-Amaro, R.; Nicaud, J.M.; Certik, M.; Rossignol, T.
    Overexpression of diacylglycerol acyltransferase in Yarrowia lipolytica affects lipid body size, number and distribution (2016), FEMS Yeast Res., 16, fow062 .
    View publication on PubMed
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene YlDGA1, overexpression in DGAT-deleted Yarrowia lipolytica strain Q4 (JMY1877), subcloning in Escherichia coli Yarrowia lipolytica
gene YlDGA2, overexpression in DGAT-deleted Yarrowia lipolytica strain Q4 (JMY1877), subcloning in Escherichia coli Yarrowia lipolytica

Protein Variants

Protein Variants Comment Organism
additional information the Q4 strain, in which the four acyltransferases have been deleted, is unable to accumulate lipids and to form lipid bodies (LBs). The expression of a single acyltransferase in this strain restores TAG accumulation and LB formation. Using this system, it becomes possible to characterize the activity and specificity of an individual DGAT. The effects of DGAT overexpression - isozymes DGAT1 or DGAT2 - on lipid accumulation and LB formation in Yarrowia lipolytica is analyzed. Overexpression of YlDGA2 in a Q4 context under neosynthesis conditions causes the formation of large lipid bodies Yarrowia lipolytica
additional information the Q4 strain, in which the four acyltransferases have been deleted, is unable to accumulate lipids and to form lipid bodies (LBs). The expression of a single acyltransferase in this strain restores TAG accumulation and LB formation. Using this system, it becomes possible to characterize the activity and specificity of an individual DGAT. The effects of DGAT overexpression - isozymes DGAT1 or DGAT2 - on lipid accumulation and LB formation in Yarrowia lipolytica is analyzed. The overexpression of YlDGA1 generates smaller but more numerous lipid bodies, a phenotype which can be enhanced by increasing the copy number of the overexpressed gene Yarrowia lipolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
 Yarrowia lipolytica 5789
-
lipid droplet
-
 Yarrowia lipolytica 5811
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol Yarrowia lipolytica
-
 CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol Yarrowia lipolytica CLIB 122
-
 CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol Yarrowia lipolytica E 150
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica A0A1H6PYY9 i.e. Candida lipolytica
-
Yarrowia lipolytica Q6C9V5 i.e. Candida lipolytica
-
Yarrowia lipolytica CLIB 122 Q6C9V5 i.e. Candida lipolytica
-
Yarrowia lipolytica E 150 Q6C9V5 i.e. Candida lipolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Yarrowia lipolytica CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Yarrowia lipolytica CLIB 122 CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Yarrowia lipolytica E 150 CoA + 1,2,3-triacylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
DGAT
-
 Yarrowia lipolytica
DGAT1
-
 Yarrowia lipolytica
DGAT2
-
 Yarrowia lipolytica
diacylglycerol acyltransferase
-
 Yarrowia lipolytica

General Information

General Information Comment Organism
malfunction overexpression of YlDGA2 in a Q4 context under neosynthesis conditions causes the formation of large lipid bodies (LBs). DGAT overexpression affects LB segregation Yarrowia lipolytica
malfunction the overexpression of YlDGA1 generates smaller but more numerous lipid bodies (LBs), a phenotype which can be enhanced by increasing the copy number of the overexpressed gene. DGAT overexpression affects LB segregation Yarrowia lipolytica
metabolism in the oleaginous yeast Yarrowia lipolytica, the diacylglycerol acyltransferases (DGATs) are major factors for triacylglycerol (TAG) synthesis Yarrowia lipolytica