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Literature summary for 2.3.1.20 extracted from

  • Roettig, A.; Strittmatter, C.S.; Schauer, J.; Hiessl, S.; Poehlein, A.; Daniel, R.; Steinbuechel, A.
    Role of wax ester synthase/acyl coenzyme A diacylglycerol acyltransferase in oleaginous Streptomyces sp. strain G25 (2016), Appl. Environ. Microbiol., 82, 5969-5981 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene atfG25, DNA and amino acid sequence determination and analysis, geneomic structure, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain C41(DE3) Streptomyces sp. G25

Protein Variants

Protein Variants Comment Organism
additional information generation of an atfG25OMEGAApr insertion mutant of Streptomyces sp. G25 Streptomyces sp. G25

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol Streptomyces sp. G25
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. G25
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain C41(DE3) by ultracentrifugation and nickel affinity chromatography Streptomyces sp. G25

Source Tissue

Source Tissue Comment Organism Textmining
additional information Streptomyces strain G25 can accumulate extraordinarily large amounts of triacylglycerol (TAG) and consists of 64% fatty acids (dry weight) when cultivated with glucose and 50% fatty acids (dry weight) when cultivated with cellobiose Streptomyces sp. G25
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Streptomyces sp. G25 CoA + 1,2,3-triacylglycerol
-
 ?
lauryl-CoA + 1,2-dipalmitoyl-sn-glycerol
-
 Streptomyces sp. G25 CoA + 1,2-dipalmitoyl-3-lauryl-glycerol
-
 ?
additional information the purified enzyme AtfG25 shows acyltransferase activity with C12- or C16-acyl-CoA, C12 to C18 alcohols (ethanol, butanol, hexanol, octanol, decanol, dodecanol, tetradecanol, hexadecanol, palmitoleyl alcohol, and octadecanol, cf. EC 2.3.1.75), and dipalmitoyl glycerol. Substrate specificity, overview. The DGAT activity of AtfG25 corresponds to about 70% of its wax synthase activity Streptomyces sp. G25 ?
-
-
palmitoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
-
 Streptomyces sp. G25 CoA + 1,2,3-tripalmitoylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
AtfG25
-
 Streptomyces sp. G25
More see also EC 2.3.1.75 Streptomyces sp. G25
wax ester synthase/acyl CoA:diacylglycerol acyltransferase
-
 Streptomyces sp. G25
wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
 Streptomyces sp. G25
WS/DGAT
-
 Streptomyces sp. G25

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptomyces sp. G25

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Streptomyces sp. G25

General Information

General Information Comment Organism
malfunction the respective gene, atfG25, is inactivated in Streptomyces sp. strain G25, cells of the insertion mutant still exhibit DGAT activity and are able to store TAG, albeit in lower quantities and at lower rates than the wild-type strain Streptomyces sp. G25
metabolism the final step in triacylglycerol (TAG) biosynthesis is catalyzed by diacylglycerol acyltransferase (DGAT) Streptomyces sp. G25
physiological function enzyme AtfG25 has an important, but not exclusive, role in triacylglycerol (TAG) biosynthesis in the Streptomyces sp. G25 isolate, suggesting the presence of alternative metabolic pathways for lipid accumulation Streptomyces sp. G25