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Literature summary for 2.3.1.20 extracted from

  • Villa, J.A.; Cabezas, M.; de la Cruz, F.; Moncalian, G.
    Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity (2014), Appl. Environ. Microbiol., 80, 1132-1141.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Marinobacter nauticus

Protein Variants

Protein Variants Comment Organism
D145A the mutant is 5fold less active with 1,2-dipalmitoyl-sn-glycerol compared to the wild type enzyme Marinobacter nauticus
D271A the mutant shows reduced activity compared to the wild type enzyme Marinobacter nauticus
H140A the mutant is 10fold less active with 1,2-dipalmitoyl-sn-glycerol compared to the wild type enzyme Marinobacter nauticus
H141A the mutant is 5fold less active with 1,2-dipalmitoyl-sn-glycerol compared to the wild type enzyme Marinobacter nauticus
L119A the mutant shows reduced activity compared to the wild type enzyme Marinobacter nauticus
N270A the mutant shows reduced activity compared to the wild type enzyme Marinobacter nauticus
R305A the mutant shows reduced activity compared to the wild type enzyme Marinobacter nauticus
W120A inactive Marinobacter nauticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 x * 100000, SDS-PAGE Marinobacter nauticus

Organism

Organism UniProt Comment Textmining
Marinobacter nauticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and S200 gel filtration Marinobacter nauticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows very low activity with ethanol, methanol, butanol, and glycerol Marinobacter nauticus ?
-
 ?
palmitoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
-
 Marinobacter nauticus CoA + tripalmitoylglycerol
-
 ?
palmitoyl-CoA + hexadecanol
-
 Marinobacter nauticus hexadecyl palmitate + CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Marinobacter nauticus

Synonyms

Synonyms Comment Organism
acyl coenzyme A:diacylglycerol acyltransferase
-
 Marinobacter nauticus
Ma2
-
 Marinobacter nauticus