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Literature summary for 2.3.1.20 extracted from
- Key enzymes for biosynthesis of neutral lipid storage compounds in prokaryotes: Properties, function and occurrence of wax ester synthases/acyl-CoA:diacylglycerol acyltransferases (2007), Biochimie, 89, 230-242.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Mycolicibacterium smegmatis |
| expressed in Escherichia coli | Streptomyces avermitilis |
| expressed in Escherichia coli | Acinetobacter baylyi |
| expressed in Escherichia coli | Mycobacterium tuberculosis |
| expressed in Escherichia coli | Streptomyces coelicolor |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a SCO0958 deletion mutant shows that the protein is responsible for biosynthesis of a significant amount of triacylglycerol | Streptomyces coelicolor |
| additional information | expression of atfA in a quadruple mutant of Saccharomyces cerevisiae, lacking own DGAT and steryl ester synthase activites by disrupted DGA1, LRO1, ARE1 and ARE2, restores triacylglycerol but not steryl ester biosynthesis and results in the formation and accumulation of fatty acid ethyl and isoamyl esters, indicating that also eukaryotic systems are suitable hosts for atfA expression | Acinetobacter baylyi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
oleoyl-CoA | - |
Mycobacterium tuberculosis |  |
| 0.028 | - |
diolein | - |
Mycobacterium tuberculosis | |
| 0.667 | - |
oleoyl-CoA | - |
Mycobacterium tuberculosis | |
| 0.714 | - |
diolein | - |
Mycobacterium tuberculosis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | protein is mainly localised on the cytoplasmic site of the plasma membrane and on the surface of intracellular wax ester inclusions | Acinetobacter baylyi | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30800 | - |
deduced from cDNA | Mycobacterium tuberculosis |
| 47100 | - |
deduced from cDNA | Streptomyces coelicolor |
| 47400 | - |
deduced from cDNA | Streptomyces avermitilis |
| 47400 | - |
deduced from cDNA | Acinetobacter baylyi |
| 49300 | - |
deduced from cDNA | Mycobacterium tuberculosis |
| 49600 | - |
Wdh3563-2 deduced from cDNA | Mycolicibacterium smegmatis |
| 49900 | - |
Wdh3563-4 deduced from cDNA | Mycolicibacterium smegmatis |
| 50200 | - |
Wdh3563-1 deduced from cDNA | Mycolicibacterium smegmatis |
| 50700 | - |
deduced from cDNA | Mycobacterium tuberculosis |
| 50900 | - |
deduced from cDNA | Mycobacterium tuberculosis |
| 51600 | - |
Wdh3563-7 deduced from cDNA | Mycolicibacterium smegmatis |
| 51900 | - |
Wdh3563-3 deduced from cDNA | Mycolicibacterium smegmatis |
| 97000 | - |
gel filtration | Acinetobacter baylyi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter baylyi | Q8GGG1 | - |
- |
| Mycobacterium tuberculosis | P9WKC3 | - |
- |
| Mycobacterium tuberculosis | P9WKC5 | - |
- |
| Mycobacterium tuberculosis | P9WKC7 | - |
- |
| Mycobacterium tuberculosis | P9WKC9 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WKC3 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WKC5 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WKC7 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WKC9 | - |
- |
| Mycolicibacterium smegmatis | - |
- |
- |
| Streptomyces avermitilis | Q826D7 | - |
- |
| Streptomyces coelicolor | Q9RIU8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified enzyme is obtained by cation exchange chromatography, hydrophobic interaction chromatography and subsequent ion exchange chromatography | Acinetobacter baylyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
does not show any DGAT activity when expressed in recombinant Escherichia coli | Streptomyces coelicolor |
| additional information | - |
high DGAT activity when expressed in recombinant Escherichia coli | Mycobacterium tuberculosis |
| additional information | - |
shows DGAT activity when expressed in recombinant Escherichia coli | Mycolicibacterium smegmatis |
| additional information | - |
shows DGAT activity when expressed in recombinant Escherichia coli | Streptomyces avermitilis |
| 0.3 | - |
- |
Acinetobacter baylyi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, without significant loss of its activity | Acinetobacter baylyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | ATfA exhibits a clear preference for the acylation of the sn-2 position of sn-1,2-dipalmitoylglycerol rather than the sn-2 position of sn-1,3-dipalmitoylglycerol | Acinetobacter baylyi | ? | - |
? | |
| oleoyl-CoA + diolein | - |
Mycobacterium tuberculosis | CoA + triolein | - |
? | |
| oleoyl-CoA + diolein | - |
Mycobacterium tuberculosis H37Rv | CoA + triolein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Acinetobacter baylyi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtfA | enzyme possesses both wax ester synthase and diacylglycerol:acyl-coenzyme A acyltransferase (DGAT) activities | Acinetobacter baylyi |
| DGAT | - |
Mycolicibacterium smegmatis |
| DGAT | - |
Streptomyces avermitilis |
| DGAT | - |
Acinetobacter baylyi |
| DGAT | - |
Mycobacterium tuberculosis |
| DGAT | - |
Streptomyces coelicolor |
| diacylglycerol acyltransferase | - |
Mycolicibacterium smegmatis |
| diacylglycerol acyltransferase | - |
Streptomyces avermitilis |
| diacylglycerol acyltransferase | - |
Acinetobacter baylyi |
| diacylglycerol acyltransferase | - |
Mycobacterium tuberculosis |
| diacylglycerol acyltransferase | - |
Streptomyces coelicolor |
| Rv3088 (TGS4) | - |
Mycobacterium tuberculosis |
| Rv3130c (TGS1) | - |
Mycobacterium tuberculosis |
| Rv3234c (TGS3) | - |
Mycobacterium tuberculosis |
| Rv3734 (TGS2) | - |
Mycobacterium tuberculosis |
| SAV7256 | enzyme possesses both wax ester synthase and diacylglycerol:acyl-coenzyme A acyltransferase (DGAT) activities | Streptomyces avermitilis |
| SCO0958 | - |
Streptomyces coelicolor |
| TAG synthase | - |
Mycobacterium tuberculosis |
| Wdh3563-1 | - |
Mycolicibacterium smegmatis |
| Wdh3563-2 | - |
Mycolicibacterium smegmatis |
| Wdh3563-3 | - |
Mycolicibacterium smegmatis |
| Wdh3563-4 | - |
Mycolicibacterium smegmatis |
| Wdh3563-7 | - |
Mycolicibacterium smegmatis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | - |
Acinetobacter baylyi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
total loss of activity at temperatures above 65°C | Acinetobacter baylyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Acinetobacter baylyi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.2 | - |
Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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