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Literature summary for 2.3.1.20 extracted from

  • Polokoff, M.A.; Bell, R.M.
    Solubilization, partial purification and characterization of rat liver microsomal diacylglycerol acyltransferase (1980), Biochim. Biophys. Acta, 618, 129-142.
    View publication on PubMed
Search for more literature for 2.3.1.20

Activating Compound

Activating Compound Comment Organism Structure
phosphocholine from soy bean, activation Rattus norvegicus
Phospholipid microsomal Rattus norvegicus

General Stability

General Stability Organism
MgCl2 accelerates loss of activity during storage at 4°C, dilution increases stability, cholate-solubilized activity is more stable than deoxycholate-solubilized activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
cholate not up to 4 mM Rattus norvegicus
palmitoyl-CoA high concentration Rattus norvegicus
Triton X-100 above 0.05 mM Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strongly dependent Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus
with Triton X-100, deoxycholate or cholate Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0024 0.038
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, at least 24 h, deoxycholate solubilized enzyme Rattus norvegicus
-80°C, undiluted, several weeks, cholate solubilized enzyme Rattus norvegicus
4°C, 20-30% loss of activity after 24 h, cholate solubilized enzyme, dilution restores activity Rattus norvegicus
4°C, about 65-85% loss of activity after 24 h, deoxycholate solubilized enzyme, partially restorable by dialysis against buffer lacking detergent Rattus norvegicus
4°C, inactivation after 24 h in the presence of 8 mM MgCl2, deoxycholate solubilized enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Rattus norvegicus CoA + 1,2-dioleoyl-3-palmitoylglycerol
-
 ?