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Literature summary for 2.3.1.199 extracted from
- Engineering and mechanistic studies of the Arabidopsis FAE1 beta-ketoacyl-CoA synthase, FAE1 KCS (2002), Eur. J. Biochem., 269, 3531-3539.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae strain InvSc1 | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C223A | the mutant enzyme lacking the acylation site is unable to carry out decarboxylation of malonyl-CoA even when oleic acid-CoA is present | Arabidopsis thaliana |
| H391A | the mutant enzyme lacking the acylation site is unable to carry out decarboxylation of malonyl-CoA even when oleic acid-CoA is present | Arabidopsis thaliana |
| H391K | the mutant shows very low condensation activity | Arabidopsis thaliana |
| H391Q | the mutant shows low condensation activity (25% activity compared to the wild type enzyme) | Arabidopsis thaliana |
| N424D | the mutant shows low condensation activity | Arabidopsis thaliana |
| N424H | the mutant enzyme lacking the acylation site is unable to carry out decarboxylation of malonyl-CoA even when oleic acid-CoA is present | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Arabidopsis thaliana | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-PDC column chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidoyl-CoA + malonyl-CoA | 25% activity compared to oleoyl-CoA | Arabidopsis thaliana | ? | - |
? |  |
| additional information | the enzyme shows highest activity towards saturated and monounsaturated C16 and C18. In the absence of an acyl-CoA substrate, the enzyme is unable to carry out decarboxylation of malonyl-CoA | Arabidopsis thaliana | ? | - |
? | |
| additional information | the enzyme shows no activity with polyunsaturated linoleic acid and alpha-linolenic acid and little or no activity with acyl-CoAs having 22 carbons or longer in chain length | Arabidopsis thaliana | ? | - |
? | |
| oleoyl-CoA + malonyl-CoA | preferred substrate | Arabidopsis thaliana | CoA + 3-oxo-eicosenoyl-CoA + 3-oxo-erucoyl-CoA + CO2 | 3-oxo-eicosenoyl-CoA is the major product | ? | |
| palmitoleoyl-CoA + malonyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
| palmitoyl-CoA + malonyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
| stearoyl-CoA + malonyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAE1 beta-ketoacyl-CoA synthase | - |
Arabidopsis thaliana |
| FAE1 KCS | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7.5 | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | CoA, NADPH and ATP have no effect on the condensation activity of the recombinant enzyme | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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