BRENDA - Enzyme Database show
show all sequences of 2.3.1.199

Substrate specificity of Arabidopsis 3-ketoacyl-CoA synthases

Blacklock, B.; Jaworski, J.; Biochem. Biophys. Res. Commun. 346, 583-590 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
ecotype Columbia
-
Purification (Commentary)
Commentary
Organism
the His6-tagged enzyme is purified by Ni2+-pentadentate chelator affinity matrix column chromatography
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arachidoyl-CoA + malonyl-CoA
best substrate for At4g34510
718730
Arabidopsis thaliana
?
-
-
-
?
additional information
At1g01120 (KCS1) and At2g26640 have broad substrate specificities when assayed with saturated and monounsaturated C16 to C24 acyl-CoAs while At4g34510 is specific for saturated fatty acyl-CoA substrates. KCS1 is most active with palmitic acid, palmitoleic acid, stearic acid, and arachidic acid but has very little activity with oleic acid and acyl chains longer than C20. At4g34510 is specific for saturated acyl-CoAs up to C22. FAE1 uses palmitic acid, palmitoleic acid, stearic acid, oleic acid, arachidic acid, 20:1, behenic acid, erucic acid, and lignoceric acid as substratesis and is specific for acyl-CoAs of 16 and 18 carbons in length
718730
Arabidopsis thaliana
?
-
-
-
-
palmitoyl-CoA + malonyl-CoA
best substrate for KCS1
718730
Arabidopsis thaliana
?
-
-
-
?
stearoyl-CoA + malonyl-CoA
best substrate for At2g26640 and FAE1
718730
Arabidopsis thaliana
?
-
-
-
?
very-long-chain acyl-CoA + malonyl-CoA
-
718730
Arabidopsis thaliana
CoA + very-long-chain 3-oxoacyl-CoA + CO2
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
the His6-tagged enzyme is purified by Ni2+-pentadentate chelator affinity matrix column chromatography
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arachidoyl-CoA + malonyl-CoA
best substrate for At4g34510
718730
Arabidopsis thaliana
?
-
-
-
?
additional information
At1g01120 (KCS1) and At2g26640 have broad substrate specificities when assayed with saturated and monounsaturated C16 to C24 acyl-CoAs while At4g34510 is specific for saturated fatty acyl-CoA substrates. KCS1 is most active with palmitic acid, palmitoleic acid, stearic acid, and arachidic acid but has very little activity with oleic acid and acyl chains longer than C20. At4g34510 is specific for saturated acyl-CoAs up to C22. FAE1 uses palmitic acid, palmitoleic acid, stearic acid, oleic acid, arachidic acid, 20:1, behenic acid, erucic acid, and lignoceric acid as substratesis and is specific for acyl-CoAs of 16 and 18 carbons in length
718730
Arabidopsis thaliana
?
-
-
-
-
palmitoyl-CoA + malonyl-CoA
best substrate for KCS1
718730
Arabidopsis thaliana
?
-
-
-
?
stearoyl-CoA + malonyl-CoA
best substrate for At2g26640 and FAE1
718730
Arabidopsis thaliana
?
-
-
-
?
very-long-chain acyl-CoA + malonyl-CoA
-
718730
Arabidopsis thaliana
CoA + very-long-chain 3-oxoacyl-CoA + CO2
-
-
-
?
Other publictions for EC 2.3.1.199
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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1
1
1
1
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737127
Naganuma
Two modes of regulation of the ...
Homo sapiens
PLoS ONE
9
e101823
2014
2
-
1
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1
1
1
2
1
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1
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1
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1
1
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1
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2
2
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736796
Sassa
Impaired epidermal permeabilit ...
Mus musculus
Mol. Cell. Biol.
33
2787-2796
2013
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736971
Tsuda
ONION2 fatty acid elongase is ...
Oryza sativa
Plant Cell Physiol.
54
209-217
2013
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1
1
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720637
Tresch
Inhibition of saturated very-l ...
Arabidopsis thaliana, Lemna aequinoctialis
Phytochemistry
76
162-171
2012
-
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1
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6
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6
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6
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1
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6
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6
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720673
Reina-Pinto
Misexpression of fatty acid el ...
Arabidopsis thaliana
Plant Cell
21
1252-1272
2009
-
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1
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4
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1
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2
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720703
Lee
Two Arabidopsis 3-ketoacyl CoA ...
Arabidopsis thaliana
Plant J.
60
462-475
2009
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2
1
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690742
Blacklock
A fatty acid elongase ELO with ...
Dictyostelium discoideum, Dictyostelium discoideum AX3
Biochem. Biophys. Res. Commun.
374
226-230
2008
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704734
Fujisawa
Enrichment of carotenoids in f ...
Crambe hispanica subsp. abyssinica
J. Biosci. Bioeng.
105
636-641
2008
-
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1
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720125
Qin
Genetic and biochemical studie ...
Gossypium hirsutum
J. Exp. Bot.
58
473-481
2007
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1
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1
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4
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718730
Blacklock
Substrate specificity of Arabi ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
346
583-590
2006
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719484
Katavic
Gaining insight into the role ...
Brassica napus
FEBS Lett.
562
118-124
2004
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1
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9
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719400
Ghanevati
Engineering and mechanistic st ...
Arabidopsis thaliana
Eur. J. Biochem.
269
3531-3539
2002
-
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1
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6
-
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1
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1
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3
-
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1
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1
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7
1
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1
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1
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1
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6
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7
1
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1
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718807
Kunst
Expression of the wax-specific ...
Arabidopsis thaliana
Biochem. Soc. Trans.
28
651-654
2000
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720670
Fiebig
Alterations in CER6, a gene id ...
Arabidopsis thaliana
Plant Cell
12
2001-2008
2000
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720669
Millar
CUT1, an Arabidopsis gene requ ...
Arabidopsis thaliana
Plant Cell
11
825-838
1999
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2
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720698
Todd
KCS1 encodes a fatty acid elon ...
Arabidopsis thaliana
Plant J.
17
119-130
1999
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1
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2
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487614
Dittrich
Fatty acid elongation in yeast ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae X2180-1A
Eur. J. Biochem.
252
477-485
1998
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1
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1
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1
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719791
Oh
ELO2 and ELO3, homologues of t ...
Saccharomyces cerevisiae
J. Biol. Chem.
272
17376-17384
1997
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2
2
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720697
Millar
Very-long-chain fatty acid bio ...
Arabidopsis thaliana
Plant J.
12
121-131
1997
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1
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1
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5
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1
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1
1
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719787
Toke
Isolation and characterization ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae W3031B
J. Biol. Chem.
271
18413-18422
1996
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