Cloned (Comment) | Organism |
---|---|
gene SlGPAT6, located on chromosome 9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of SlGPAT6 wild-type and DELTASlGPAT6 mutant carrying the G163R mutation in a Saccharomyces cerevisiae gat1DELTA mutant strain with a GAT1 knockout | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
G163R | isolation of a set of fruit brightness mutants (named glossy mutants) with abnormal fruit cutin amounts/compositions from the EMS-mutagenized tomato mutant population (cv Micro-Tom). The glossy mutant line P23F12 exhibits enhanced fruit brightness and a reduced cutin load. Whole-genome sequencing and gene mapping show that a single G613A nucleotide transition in the first exon leading to a G163R nonsynonymous mutation in gene SlGPAT (Solyc09g014350) is responsible for the mutant glossy fruit phenotype, i.e. mutant gpat6-a, overview. The inactive mutant gpat6-a has a fruit cuticle that shows reduced levels of C16 cutin monomers. No differences are observed in total wax load between wild-type and gpat6-a fruits, but differences in wax composition are apparent, such as increased or decreased levels of several minor C25 to C30 alkanes in the gpat6-a mutant. Since both even- and odd-numbered alkanes display similar trends, these variations are likely independent of the alkane biosynthetic pathways. The most striking wax compositional change in the gpat6-a mutant is an approximately 5fold increase in delta- and alpha-amyrin levels and to a lesser extent in beta-amyrin levels. In contrast to waxes, the total cutin load in gpat6-a is reduced by almost 3fold. MapMan-based general overview of genes differentially expressed between wild-type and gpat6-a mutant fruit | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Solanum lycopersicum | 5783 | - |
membrane | a transmembrane enzyme | Solanum lycopersicum | 16020 | - |
microsome | - |
Solanum lycopersicum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
9(10),16-dihydroxyhexadecanoyl-CoA + sn-glycerol 3-phosphate | Solanum lycopersicum | the principal putative substrate of SlGPAT6 is 9(10),16-dihydroxyhexadecanoic acid (C16:0 diOH) is the major cutin monomer present in tomato fruit cuticles | CoA + 2-(9(10),16-dihydroxyhexadecanoyl)-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | Solanum lycopersicum | - |
CoA + 2-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | A0A3Q7I0S2 | cv. Micro-Tom, a EMS-mutagenized tomato mutant population | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
16-hydroxyhexadecanoyl-CoA + sn-glycerol 3-phosphate | - |
Solanum lycopersicum | CoA + 2-(16-hydroxyhexadecanoyl)-sn-glycerol 3-phosphate | - |
? | |
9(10),16-dihydroxyhexadecanoyl-CoA + sn-glycerol 3-phosphate | - |
Solanum lycopersicum | CoA + 2-(9(10),16-dihydroxyhexadecanoyl)-sn-glycerol 3-phosphate | - |
? | |
9(10),16-dihydroxyhexadecanoyl-CoA + sn-glycerol 3-phosphate | the principal putative substrate of SlGPAT6 is 9(10),16-dihydroxyhexadecanoic acid (C16:0 diOH) is the major cutin monomer present in tomato fruit cuticles | Solanum lycopersicum | CoA + 2-(9(10),16-dihydroxyhexadecanoyl)-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | - |
Solanum lycopersicum | CoA + 2-acyl-sn-glycerol 3-phosphate | - |
? | |
hexadecane-1,16-dioyl-CoA + sn-glycerol 3-phosphate | - |
Solanum lycopersicum | CoA + 2-hexadecane-1,16-dioyl-sn-glycerol 3-phosphate | - |
? | |
hexadecanoyl-CoA + sn-glycerol 3-phosphate | - |
Solanum lycopersicum | CoA + 2-hexadecanoyl-sn-glycerol 3-phosphate | - |
? | |
additional information | analysis of SlGPAT6 substrate specificity of wild-type and mutant enzymes, overview | Solanum lycopersicum | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
GPAT6 | - |
Solanum lycopersicum |
SlGPAT6 | - |
Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
malfunction | the G163R mutation in gpat6-a confers a loss of SlGPAT6-mediated GPAT activity leading to P23F12 glossy mutant line with with abnormal fruit cutin amounts/compositions and increased fruit brightness. Loss of SlGPAT6 function leads to altered expression of genes involved in cuticle and cell wall formation and remodeling | Solanum lycopersicum |
additional information | structural modeling of SlGPAT6, based on the Methanococcus jannaschii phospho-Ser phosphatase template and restricted to the N-terminal 17 to 207 amino acid region, generated in silico, revealing that the G-to-R amino acid substitution at position 163 is close to the cluster of residues that are essential for the catalytic activity and Mg2+ binding of GPAT6 | Solanum lycopersicum |
physiological function | glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis. Wild-type SlGPAT6 has the dual acyltransferase and phosphatase activities expected from a GPAT enzyme involved in cutin monomer biosynthesis | Solanum lycopersicum |