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Literature summary for 2.3.1.197 extracted from

  • Pfoestl, A.; Hofinger, A.; Kosma, P.; Messner, P.
    Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose in Aneurinibacillus thermoaerophilus L420-91T (2003), J. Biol. Chem., 278, 26410-26417.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Aneurinibacillus thermoaerophilus

General Stability

General Stability Organism
to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4°C or -20°C and supplemented with 50% glycerol Aneurinibacillus thermoaerophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
acetyl-CoA pH 7.4, 37°C Aneurinibacillus thermoaerophilus
0.0667
-
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose pH 7.4, 37°C Aneurinibacillus thermoaerophilus

Organism

Organism UniProt Comment Textmining
Aneurinibacillus thermoaerophilus Q6T1W7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aneurinibacillus thermoaerophilus

Storage Stability

Storage Stability Organism
4°C, the concentrated enzyme preparations are stable at 4°C for several months with only a marginal decrease of enzyme activity Aneurinibacillus thermoaerophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose the enzyme is involved in the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose. The reverse reaction cannot be detected Aneurinibacillus thermoaerophilus CoA + dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactopyranose
-
?

Synonyms

Synonyms Comment Organism
dTDP-D-Fucp3N acetylase
-
Aneurinibacillus thermoaerophilus
FdtC
-
Aneurinibacillus thermoaerophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aneurinibacillus thermoaerophilus
50 55
-
Aneurinibacillus thermoaerophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose pH 7.4, 37°C Aneurinibacillus thermoaerophilus
3.1
-
acetyl-CoA pH 7.4, 37°C Aneurinibacillus thermoaerophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Aneurinibacillus thermoaerophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4
-
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose pH 7.4, 37°C Aneurinibacillus thermoaerophilus
50.8
-
acetyl-CoA pH 7.4, 37°C Aneurinibacillus thermoaerophilus