Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Aneurinibacillus thermoaerophilus |
General Stability | Organism |
---|---|
to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4°C or -20°C and supplemented with 50% glycerol | Aneurinibacillus thermoaerophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
acetyl-CoA | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus | |
0.0667 | - |
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aneurinibacillus thermoaerophilus | Q6T1W7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aneurinibacillus thermoaerophilus |
Storage Stability | Organism |
---|---|
4°C, the concentrated enzyme preparations are stable at 4°C for several months with only a marginal decrease of enzyme activity | Aneurinibacillus thermoaerophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose | the enzyme is involved in the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose. The reverse reaction cannot be detected | Aneurinibacillus thermoaerophilus | CoA + dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dTDP-D-Fucp3N acetylase | - |
Aneurinibacillus thermoaerophilus |
FdtC | - |
Aneurinibacillus thermoaerophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aneurinibacillus thermoaerophilus |
50 | 55 | - |
Aneurinibacillus thermoaerophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus | |
3.1 | - |
acetyl-CoA | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Aneurinibacillus thermoaerophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus | |
50.8 | - |
acetyl-CoA | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus |