Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, the coding region of At3g03480 is cloned into a vector that introduces an N-terminal 6His tag | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, strong inhibition | Arabidopsis thaliana | |
Co2+ | 5 mM, strong inhibition | Arabidopsis thaliana | |
Cu2+ | 5 mM, strong inhibition | Arabidopsis thaliana | |
Mg2+ | 5 mM, strong inhibition | Arabidopsis thaliana | |
Zn2+ | 5 mM, strong inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
acetyl-CoA | pH 7.7, 25°C, native enzyme, partially purified | Arabidopsis thaliana | |
0.0385 | - |
acetyl-CoA | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
0.126 | - |
(3Z)-hex-3-en-ol | pH 7.7, 25°C, native enzyme, partially purified | Arabidopsis thaliana | |
0.174 | - |
1-Octanol | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
0.251 | - |
(3Z)-hex-3-en-ol | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
native plant protein, gel filtration | Arabidopsis thaliana |
50400 | - |
1 * 50400, native plant protein, calculated from sequence | Arabidopsis thaliana |
51000 | - |
His-tagged protein, gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (3Z)-hex-3-en-ol | Arabidopsis thaliana | the major volatile released upon mechanical wounding of the leaves is (Z)-3-hexen-1-yl acetate | (3Z)-hex-3-en-1-yl acetate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SRQ2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | enzyme activity in the leaves following wounding increases over time, but this response lagges significantly behind (Z)-3-hexen-1-yl acetate release. A maximum specific activity is reached 12 h after the initial wounding event. CHAT enzyme activity declines by 35% over the course of the next 12 h | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (3Z)-hex-3-en-ol | the major volatile released upon mechanical wounding of the leaves is (Z)-3-hexen-1-yl acetate | Arabidopsis thaliana | (3Z)-hex-3-en-1-yl acetate + CoA | - |
? | |
acetyl-CoA + (3Z)-hex-3-en-ol | the enzyme has the ability to accept several medium-chain-length aliphatic and benzyl-derived alcohols, but has highest catalytic efficiency with (Z)-3-hexen-1-ol. The catalytic efficiency with 1-octanol is 0.2% of the catalytic efficiency with (Z)-3-hexen-1-ol | Arabidopsis thaliana | (3Z)-hex-3-en-1-yl acetate + CoA | - |
? | |
acetyl-CoA + 1-octanol | the enzyme has the ability to accept several medium-chain-length aliphatic and benzyl-derived alcohols, but has highest catalytic efficiency with (Z)-3-hexen-1-ol. The catalytic efficiency with 1-octanol is 0.2% of the catalytic efficiency with (Z)-3-hexen-1-ol | Arabidopsis thaliana | octanyl acetate + CoA | - |
? | |
additional information | no activity with malonyl CoA and benzoyl CoA | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50400, native plant protein, calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
acetyl CoA:(Z)-3-hexen-1-ol acetyltransferase | - |
Arabidopsis thaliana |
At3g03480 | - |
Arabidopsis thaliana |
ChAT | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min, stable | Arabidopsis thaliana |
37 | - |
30 min, 13% loss of activity | Arabidopsis thaliana |
50 | - |
30 min, complete loss of activity | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
1-Octanol | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
11.3 | - |
(3Z)-hex-3-en-ol | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
23 | - |
acetyl-CoA | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | 7.3 | - |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 9 | pH 6.3: 89% of maximal activity, pH 9.0: about 45% of maximal activity | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | RNAi transgenic plants exhibiting a severe reduction in At3g03480 transcript levels emitt no (Z)-3-hexen-1-yl acetate | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
1-Octanol | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
45 | - |
(3Z)-hex-3-en-ol | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
597 | - |
acetyl-CoA | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana |