Application | Comment | Organism |
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synthesis | engineering a budC acoABCD double mutant, which has lost 2,3-butanediol dehydrogenase activity and the acetoin dehydrogenase system for R-acetoin production. Optimized conditions include aerobic batch culture and mildly acidic conditions. 62.3 g per l R-acetoin can be produced by budC and acoABCD double mutants in 57 h culture, with an optical purity of 98.0%, and a substrate conversion ratio of 28.7% | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
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Klebsiella pneumoniae | - |
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- |
Klebsiella pneumoniae CGMCC 1.6366 | - |
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General Information | Comment | Organism |
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physiological function | the acetoin dehydrogenase enzyme system is responsible for R-acetoin dissimilation. Mutants lose the ability to grow on acetoin as the sole carbon source, and the acetoin accumulated cannot be dissimilated. In the presence of another carbon source, the acetoin accumulated in broth of acetoin dehydrogenase mutants is converted to 2,3-butanediol | Klebsiella pneumoniae |