BRENDA - Enzyme Database
show all sequences of 2.3.1.188

Formation of omega-feruloyloxypalmitic acid by an enzyme from wound-healing potato tuber disks

Lotfy, S.; Negrel, J.; Javelle, F.; Phytochemistry 35, 1419-1424 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Tris-HCl
60% of maximal activity in Tris-HCl compared with potassium phosphate buffer
Solanum tuberosum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
16-hydroxypalmitic acid
pH 7.0, 30°C
Solanum tuberosum
0.0017
-
hexadecan-1,16-diol
pH 7.0, 30°C
Solanum tuberosum
0.004
-
1-Tetradecanol
pH 7.0, 30°C
Solanum tuberosum
0.005
-
feruloyl-CoA
pH 7.0, 30°C
Solanum tuberosum
0.005
-
1-Dodecanol
pH 7.0, 30°C
Solanum tuberosum
0.0071
-
sinapoyl-CoA
pH 7.0, 30°C
Solanum tuberosum
0.0095
-
1-pentadecanol
pH 7.0, 30°C
Solanum tuberosum
0.01
-
1-hexadecanol
pH 7.0, 30°C
Solanum tuberosum
0.0143
-
p-Coumaroyl-CoA
pH 7.0, 30°C
Solanum tuberosum
0.0168
-
15-hydroxypentadecanoic acid
pH 7.0, 30°C
Solanum tuberosum
Organism
Organism
UniProt
Commentary
Textmining
Solanum tuberosum
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Solanum tuberosum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
tuber
wound-healing tuber disc
Solanum tuberosum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
feruloyl-CoA + 1-dodecanol
49% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-dodecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
feruloyl-CoA + 1-hexadecanol
52% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-hexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
feruloyl-CoA + 1-pentadecanol
53% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-pentadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
feruloyl-CoA + 1-tetradecanol
82% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-tetradecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
feruloyl-CoA + 15-hydroxypentadecanoic acid
68% of the activity with 16-hydroxypalmitate
700658
Solanum tuberosum
CoA + (E)-15-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)pentadecanoic acid
-
-
-
?
feruloyl-CoA + 16-hydroxypalmitic acid
-
700658
Solanum tuberosum
CoA + (E)-16-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
feruloyl-CoA + hexadecane-1,16-diol
52% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-16-hydroxyhexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
no formation of the diferulate
-
-
?
p-coumaroyl-CoA + 16-hydroxypalmitic acid
51% of the activity with feruloyl-CoA
700658
Solanum tuberosum
CoA + (E)-16-(3-(4-hydroxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
sinapoyl-CoA + 16-hydroxypalmitic acid
50% of the activity with feruloyl-CoA
700658
Solanum tuberosum
CoA + (E)-16-(3-(4-hydroxy-3,5-dimethoxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
potassium phosphate buffer, 60% of maximal activity in Tris-HCl
Solanum tuberosum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
-
half-maximal activity
Solanum tuberosum
7.5
-
half-maximal activity
Solanum tuberosum
10
-
5% of maximal acitvity
Solanum tuberosum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Tris-HCl
60% of maximal activity in Tris-HCl compared with potassium phosphate buffer
Solanum tuberosum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
16-hydroxypalmitic acid
pH 7.0, 30°C
Solanum tuberosum
0.0017
-
hexadecan-1,16-diol
pH 7.0, 30°C
Solanum tuberosum
0.004
-
1-Tetradecanol
pH 7.0, 30°C
Solanum tuberosum
0.005
-
feruloyl-CoA
pH 7.0, 30°C
Solanum tuberosum
0.005
-
1-Dodecanol
pH 7.0, 30°C
Solanum tuberosum
0.0071
-
sinapoyl-CoA
pH 7.0, 30°C
Solanum tuberosum
0.0095
-
1-pentadecanol
pH 7.0, 30°C
Solanum tuberosum
0.01
-
1-hexadecanol
pH 7.0, 30°C
Solanum tuberosum
0.0143
-
p-Coumaroyl-CoA
pH 7.0, 30°C
Solanum tuberosum
0.0168
-
15-hydroxypentadecanoic acid
pH 7.0, 30°C
Solanum tuberosum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Solanum tuberosum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
tuber
wound-healing tuber disc
Solanum tuberosum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
feruloyl-CoA + 1-dodecanol
49% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-dodecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
feruloyl-CoA + 1-hexadecanol
52% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-hexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
feruloyl-CoA + 1-pentadecanol
53% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-pentadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
feruloyl-CoA + 1-tetradecanol
82% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-tetradecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
feruloyl-CoA + 15-hydroxypentadecanoic acid
68% of the activity with 16-hydroxypalmitate
700658
Solanum tuberosum
CoA + (E)-15-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)pentadecanoic acid
-
-
-
?
feruloyl-CoA + 16-hydroxypalmitic acid
-
700658
Solanum tuberosum
CoA + (E)-16-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
feruloyl-CoA + hexadecane-1,16-diol
52% of the activity with 16-hydroxypalmitic acid
700658
Solanum tuberosum
CoA + (E)-16-hydroxyhexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
no formation of the diferulate
-
-
?
p-coumaroyl-CoA + 16-hydroxypalmitic acid
51% of the activity with feruloyl-CoA
700658
Solanum tuberosum
CoA + (E)-16-(3-(4-hydroxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
sinapoyl-CoA + 16-hydroxypalmitic acid
50% of the activity with feruloyl-CoA
700658
Solanum tuberosum
CoA + (E)-16-(3-(4-hydroxy-3,5-dimethoxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
potassium phosphate buffer, 60% of maximal activity in Tris-HCl
Solanum tuberosum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
-
half-maximal activity
Solanum tuberosum
7.5
-
half-maximal activity
Solanum tuberosum
10
-
5% of maximal acitvity
Solanum tuberosum
Other publictions for EC 2.3.1.188
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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254
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-
1
-
-
-
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-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
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-
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-
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-
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1
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1
1
1
1
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Purification and characterizat ...
Nicotiana tabacum
Planta
199
475-480
1996
-
-
-
-
-
-
-
9
-
-
1
-
-
1
-
-
1
-
-
1
-
-
10
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
-
1
-
1
-
-
10
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
700659
Lotfy
-
Distribution of hydroxycinnamo ...
Allium ampeloprasum, Nicotiana tabacum, Solanum tuberosum, Taraxacum officinale
Phytochemistry
40
389-391
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
8
-
8
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-
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-
-
-
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-
-
-
-
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3
8
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700658
Lotfy
-
Formation of omega-feruloyloxy ...
Solanum tuberosum
Phytochemistry
35
1419-1424
1994
-
-
-
-
-
-
1
10
-
-
-
-
-
1
-
-
1
-
-
1
-
-
9
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
10
-
-
-
-
-
-
-
1
-
1
-
-
9
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-