Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.184 extracted from

  • Rampioni, G.; Bertani, I.; Pillai, C.R.; Venturi, V.; Zennaro, E.; Leoni, L.
    Functional characterization of the quorum sensing regulator RsaL in the plant-beneficial strain Pseudomonas putida WCS358 (2012), Appl. Environ. Microbiol., 78, 726-734.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.184

Cloned(Commentary)

Cloned (Comment) Organism
gene lasI, genetic organization and las genetic locus Pseudomonas aeruginosa
gene ppuI, genetic organization and ppu genetic locus Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
9-hydroxy-2-propyl-4H-pyrido[1,2-a]pyrimidin-4-one
-
 Pseudomonas putida
N-(3-oxocyclohex-1-en-1-yl)octanamide
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine Pseudomonas aeruginosa
-
 [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine Pseudomonas putida
-
 [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine Pseudomonas putida WCS358
-
 [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 gene lasI
-
Pseudomonas putida
-
 gene ppuI
-
Pseudomonas putida WCS358
-
 gene ppuI
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
-
 Pseudomonas aeruginosa [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
-
 Pseudomonas putida [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
-
 Pseudomonas putida WCS358 [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?

Synonyms

Synonyms Comment Organism
LasI
-
 Pseudomonas aeruginosa
LasI synthase
-
 Pseudomonas aeruginosa
PppuI
-
 Pseudomonas putida

Expression

Organism Comment Expression
Pseudomonas putida RsaLWCS displays a strong repressive effect on the promoter of the quorum sensing signal synthase gene, ppuI. Purified RsaLWCS specifically binds to ppuI on a DNA region overlapping the predicted sigma70-binding site, but the RsaLWCS protein is not able to form stable complexes efficiently with a probe encompassing the ppuI promoter, the RsaLWCS/PppuI complex is not stable in vitro, RsaLWCS needs a molecular interactor/cofactor specific for Pseudomonas putida WCS358 to repress ppuI transcription. RsaLWCS regulates siderophore-mediated growth limitation of plant pathogens and biofilmcformation, two processes relevant for plant growth-promoting activity. Mutational analysis using a chimeric RsaLWCS mutant, RsaLWCS molecular mechanism, overview down
Pseudomonas aeruginosa RsaLWCS does not show a repressive effect on the promoter of the QS signal synthase gene lasI in Pseudomonas aeruginosa. RsaLWCS formed stable complexes with the promoter of lasI, the gene orthologous to ppuI. RsaLWCS regulates siderophore-mediated growth limitation of plant pathogens and biofilm formation, two processes relevant for plant growth-promoting activity. RsaL binds close to LasR on the same bidirectional promoter, repressing the transcription of both the lasI and rsaL genes. Molecular mechanism, overview down

General Information

General Information Comment Organism
physiological function quorum sensing systems rely on a signal receptor and a synthase producing N-acyl-homoserine lactone(s) as the signal molecule(s). The rsaL gene, located between the signal receptor and synthase genes, encodes a repressor limiting signal synthase expression and hence signal molecule production, molecular mechanism of action of the RsaL protein in the plant growth-promoting rhizobacterium Pseudomonas putida WCS358, overview Pseudomonas putida
physiological function quorum sensing systems rely on a signal receptor and a synthase producing N-acyl-homoserine lactone(s) as the signal molecule(s). The rsaL gene, located between the signal receptor and synthase genes, encodes a repressor limiting signal synthase expression and hence signal molecule production, molecular mechannism, overview Pseudomonas aeruginosa