Cloned (Comment) | Organism |
---|---|
gene luxI, expression as maltose-binding protein-fusion enzyme in Escherichia coli strain XL 1-Blue | Aliivibrio fischeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
hexanoyl-[acyl-carrier protein] | pH 7.5, 23°C | Aliivibrio fischeri | |
0.13 | - |
S-adenosyl-L-methionine | pH 7.5, 23°C | Aliivibrio fischeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aliivibrio fischeri | acylhomoserine lactones are autoinducers of quorum-sensing signaling | ? | - |
? | |
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] | Aliivibrio fischeri | - |
5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] | Aliivibrio fischeri | - |
5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio fischeri | - |
gene luxI | - |
Purification (Comment) | Organism |
---|---|
recombinant maltose-binding protein-fusion enzyme from Escherichia coli strain XL 1-Blue by amylose affinity chromatography | Aliivibrio fischeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acylhomoserine lactones are autoinducers of quorum-sensing signaling | Aliivibrio fischeri | ? | - |
? | |
additional information | substrate specificity of purified recombinant enzyme | Aliivibrio fischeri | ? | - |
? | |
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] | - |
Aliivibrio fischeri | 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] | - |
Aliivibrio fischeri | 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl homoserine lactone synthase | - |
Aliivibrio fischeri |
autoinducer synthase | - |
Aliivibrio fischeri |
LuxI protein | - |
Aliivibrio fischeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | - |
Aliivibrio fischeri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
18 | 37 | 57% of maximal activity at 18°C and 10% at 37°C | Aliivibrio fischeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Aliivibrio fischeri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 8 | 10% of maximal acivity at pH 7.0, 65% at pH 7.1, and 50% at pH 8.0 | Aliivibrio fischeri |