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Literature summary for 2.3.1.184 extracted from
- Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa (2005), Biochemistry, 44, 2974-2981.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rhlI, overexpression in Escherichia coli strain XL 1-Blue as maltose binding protein-fusion protein, overexpression of His-tagged RhlI in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0059 | - |
butyryl-[acyl-carrier protein 2] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa |  |
| 0.0074 | - |
butyryl-[acyl-carrier protein 1] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
| 0.2 | - |
butyryl-CoA | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
| 0.283 | - |
butyryl-[acyl-carrier protein 3] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | Pseudomonas aeruginosa | substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant maltose binding protein-fusion enzyme RhlI from Escherichia coli strain XL 1-Blue by amylose affinity chromatography, recombinant His-tagged RhlI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas aeruginosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone | structures of substrate, product, and transition state, overview | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + butyryl-CoA | substrate of RhlI, very low activity | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA | - |
? | |
| S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 1] | substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 1] | - |
? | |
| S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 2] | substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 2] | - |
? | |
| S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 3] | substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates, very low activity with ACP3 | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 3] | - |
? | |
| S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acylhomoserine lactone synthase | - |
Pseudomonas aeruginosa |
| RhlI | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
butyryl-[acyl-carrier protein 3] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
| 0.05 | - |
butyryl-CoA | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
| 0.35 | - |
butyryl-[acyl-carrier protein 1] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
| 0.46 | - |
butyryl-[acyl-carrier protein 2] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
assay at | Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH-profile | Pseudomonas aeruginosa |
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