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Literature summary for 2.3.1.183 extracted from

  • Wu, G.; Yuan, M.; Wei, L.; Zhang, Y.; Lin, Y.; Zhang, L.; Liu, Z.
    Characterization of a novel cold-adapted phosphinothricin N-acetyltransferase from the marine bacterium Rhodococcus sp. strain YM12 (2014), J. Mol. Catal. B, 104, 23-28.
No PubMed abstract available
Search for more literature for 2.3.1.183

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure. Enzyme is a dimer. Ring stacking and a salt bridge derived from residues His114-Trp152 and Arg72-Glu82, respectively, are important forces for the subunit interaction Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 L-phosphinothricin pH 8.0, 35°C Rhodococcus sp.
0.156
-
 L-methionine sulfoximine pH 8.0, 35°C Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, recombinant GST-fusion protein Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp. M1EYW6
-
-
Rhodococcus sp. YM12 M1EYW6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-methionine sulfoximine
-
 Rhodococcus sp. ?
-
 ?
acetyl-CoA + L-methionine sulfoximine
-
 Rhodococcus sp. YM12 ?
-
 ?
acetyl-CoA + L-phosphinothricin
-
 Rhodococcus sp. CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin
-
 Rhodococcus sp. YM12 CoA + L-N-acetylphosphinothricin
-
 ?
additional information no substrate: glyphosate Rhodococcus sp. ?
-
 ?
additional information no substrate: glyphosate Rhodococcus sp. YM12 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, recombinant GST-fusion protein Rhodococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Rhodococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 10 more than 50% of maximum activity Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 80 min, more than 80% residual activity Rhodococcus sp.
50
-
 100 min, 60% residual activity Rhodococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 L-methionine sulfoximine pH 8.0, 35°C Rhodococcus sp.
2.18
-
 L-phosphinothricin pH 8.0, 35°C Rhodococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
8
-
-
 Rhodococcus sp.