Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.183 extracted from

  • Yun, C.S.; Hasegawa, H.; Nanamiya, H.; Terakawa, T.; Tozawa, Y.
    Novel bacterial N-acetyltransferase gene for herbicide detoxification in land plants and selection maker in plant transformation (2009), Biosci. Biotechnol. Biochem., 73, 1000-1006.
    View publication on PubMed
Search for more literature for 2.3.1.183

Application

Application Comment Organism
agriculture creation of herbicide resistant plants Nocardia sp. AB2253
biotechnology creation of herbicide resistant plants Nocardia sp. AB2253

Cloned(Commentary)

Cloned (Comment) Organism
expressed as chimeric protein in Escherichia coli and in pea chloroplasts after transformation with Agrobacterium tumefaciens Nocardia sp. AB2253

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 L-phosphinothricin
-
 Nocardia sp. AB2253
14.31
-
 methionine sulfone
-
 Nocardia sp. AB2253

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19600
-
 SDS-PAGE Nocardia sp. AB2253

Organism

Organism UniProt Comment Textmining
Nocardia sp. AB2253 B0I560 soil isolate
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 5,5'-dithiobis-(2-nitrobenzoic acid)
-
 Nocardia sp. AB2253 CoA + 5-thio-2-nitrobenzoic acid
-
 ?
acetyl-CoA + L-phosphinothricin
-
 Nocardia sp. AB2253 CoA + L-N-acetylphosphinothricin
-
 ir
acetyl-CoA + methionine sulfone
-
 Nocardia sp. AB2253 CoA + ?
-
 ?

Synonyms

Synonyms Comment Organism
MAT
-
 Nocardia sp. AB2253
methionine sulfone N-acetyltransferase
-
 Nocardia sp. AB2253
phosphinothricin-N-acetyltransferase
-
 Nocardia sp. AB2253

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 in Tris buffer Nocardia sp. AB2253

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 Catalytic activity reaches a maximum at pH 8 to pH 8.5. In Tris buffer at pH 7 and pH 9.5 20-30% catalytic activity remains. In phosphate buffer, the activity at pH 7 and pH 6.5 is 15% and 10%, respectively. At pH 6 the enzyme shows no activity. Nocardia sp. AB2253

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Nocardia sp. AB2253

General Information

General Information Comment Organism
metabolism detoxification of the nonselective herbicide phosphinothricin, commercial named Roundup Ready, Finale or Basta Nocardia sp. AB2253