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Literature summary for 2.3.1.183 extracted from

  • Shim, Y.Y.; Shin, W.S.; Moon, G.S.; Kim, K.H.
    Quantitative analysis of phosphinothricin-N-acetyltransferase in genetically modified herbicide tolerant pepper by an enzyme-linked immunosorbent assay (2007), J. Microbiol. Biotechnol., 17, 681-684.
    View publication on PubMed
Search for more literature for 2.3.1.183

Application

Application Comment Organism
analysis an immunoassay method is developed to quantitatively detect phosphinothricin-N-acetyltransferase encoded by the Bialaphos resistance gene in genetically modified pepper Capsicum annuum

Cloned(Commentary)

Cloned (Comment) Organism
into the pQE31 vector for expression in Escherichia coli M15 cells Capsicum annuum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 determined by SDS-PAGE and Western Blot analysis Capsicum annuum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + phosphinothricin Capsicum annuum
-
 CoA + N-acetylphosphinothricin
-
 ?

Organism

Organism UniProt Comment Textmining
Capsicum annuum
-
 the bar gene is originally isolated from Streptomyces hygroscopicus and Streptomyces viridochromogenes
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant protein is purified by Ni2+ affinity chromatography and used for the production of polyclonal antibodies, for Western blot analysis the protein is extracted from peppers Capsicum annuum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + phosphinothricin
-
 Capsicum annuum CoA + N-acetylphosphinothricin
-
 ?

Synonyms

Synonyms Comment Organism
Pat
-
 Capsicum annuum
phosphinothricin-N-acetyltransferase
-
 Capsicum annuum