Literature summary for 2.3.1.183 extracted from

Davies, A.M.; Tata, R.; Beavil, R.L.; Sutton, B.J.; Brown, P.R.
l-Methionine sulfoximine, but not phosphinothricin, is a substrate for an acetyltransferase (gene PA4866) from Pseudomonas aeruginosa: structural and functional studies (2007), Biochemistry, 46, 1829-1839.

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Cloned(Commentary)

Cloned (Comment)	Organism
into the vector pET24a for expression in Escherichia coli BL21DE3 cells	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
a high-resolution, 1.55 A, crystal structure of pita in complex with L-methionine sulfoximine is solved	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
Depp4	mutant, in which pita is inactivated through an in-frame deletion	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.03	-	acetyl-CoA	apparent KM	Pseudomonas aeruginosa
1.3	-	L-methionine sulfoximine	apparent KM	Pseudomonas aeruginosa
1.3	-	L-methionine sulfone	apparent KM	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HUU7	putative uncharacterized protein	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + L-methionine sulfone	-	Pseudomonas aeruginosa	?	-	?
acetyl-CoA + L-methionine sulfoximine	enzyme has no activity towards phosphinothricin	Pseudomonas aeruginosa	?	-	?

Synonyms

Synonyms	Comment	Organism
phosphinothricin acetyltransferase	-	Pseudomonas aeruginosa
PitA	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	activity assay	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
505	-	L-methionine sulfoximine	apparent Kcat	Pseudomonas aeruginosa
610	-	L-methionine sulfone	apparent Kcat	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	activity assay	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)