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Literature summary for 2.3.1.183 extracted from
- Purification and partial characterization of the Streptomyces viridochromogenes Tue494 phosphinothricin-N-acetyltransferase mediating resistance to the herbicide phosphinothricin in transgenic plants (1995), Z. Naturforsch. C, 50, 796-805.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Streptomyces viridochromogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Streptomyces viridochromogenes |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
2 * 20000, about, recombinant enzyme | Streptomyces viridochromogenes |
| 40000 | - |
recombinant enzyme, gel filtration | Streptomyces viridochromogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Streptomyces viridochromogenes | the enzyme confers resistance to the herbicide phosphinothricin | ? | - |
? |  |
| additional information | Streptomyces viridochromogenes Tue494 | the enzyme confers resistance to the herbicide phosphinothricin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces viridochromogenes | - |
- |
- |
| Streptomyces viridochromogenes Tue494 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, anion exchange, hydrophobic interaction, and hydroxylapatite chromatography, followed by gel filtration, to about 90% purity | Streptomyces viridochromogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme confers resistance to the herbicide phosphinothricin | Streptomyces viridochromogenes | ? | - |
? | |
| additional information | the enzyme confers resistance to the herbicide phosphinothricin | Streptomyces viridochromogenes Tue494 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 20000, about, recombinant enzyme | Streptomyces viridochromogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pat | - |
Streptomyces viridochromogenes |
| phosphinothricin-N-acetyltransferase | - |
Streptomyces viridochromogenes |
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