Any feedback?
Literature summary for 2.3.1.183 extracted from
- Characterization of phosphinothricin acetyltransferase and C-terminal enzymatically active fusion proteins (1991), Gene, 102, 33-37.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | the bar gene represents a selectable and assayable reporter gene especially suitable for 3Â’-terminal gene fusions | Streptomyces hygroscopicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bar, expression in Escherichia coli with gene fragments fused to the 3' end, the fusion gene product shows unaltered kinetic properties compared to the unmodified gene product | Streptomyces hygroscopicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetic analysis of the recombinant wild-type and modified enzymes | Streptomyces hygroscopicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-phosphinothricin | Streptomyces hygroscopicus | - |
CoA + L-N-acetylphosphinothricin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces hygroscopicus | - |
gene bar | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-phosphinothricin | - |
Streptomyces hygroscopicus | CoA + L-N-acetylphosphinothricin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pat | - |
Streptomyces hygroscopicus |
| PPT acetyltransferase | - |
Streptomyces hygroscopicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | - |
Streptomyces hygroscopicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 55 | - |
Streptomyces hygroscopicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces hygroscopicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | no activity at pH 5.0 and pH 10.0 | Streptomyces hygroscopicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Streptomyces hygroscopicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
