Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
C150A | loss of the overall catalytic activity and an inability to form the acyl-enzyme intermediate | Bacillus subtilis |
C150S | loss of the overall catalytic activity and an inability to form the acyl-enzyme intermediate | Bacillus subtilis |
K165A | mutant has weakened catalytic ability | Bacillus subtilis |
K165R | mutant remains active | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-[acyl-carrier protein] + a protein | Bacillus subtilis | - |
a protein N6-(octanoyl)lysine + an [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-[acyl-carrier protein] + a protein | - |
Bacillus subtilis | a protein N6-(octanoyl)lysine + an [acyl-carrier protein] | - |
? | |
octanoyl-[acyl-carrier protein] + a protein | the transfer of the octanoyl group from octanoyl-ACP to Bacillus subtilis GcvH occurs in a LipM-dependent manner. The LipM reaction proceeds through a thioester-linked acyl enzyme intermediate. The LipM active site nucleophile is identified as C150 | Bacillus subtilis | a protein N6-(octanoyl)lysine + an [acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 14000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
LipM | - |
Bacillus subtilis |
octanoyltransferase LipM | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus subtilis |