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Literature summary for 2.3.1.181 extracted from

  • Nesbitt, N.M.; Baleanu-Gogonea, C.; Cicchillo, R.M.; Goodson, K.; Iwig, D.F.; Broadwater, J.A.; Haas, J.A.; Fox, B.G.; Booker, S.J.
    Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase (2005), Protein Expr. Purif., 39, 269-282.
    View publication on PubMed
Search for more literature for 2.3.1.181

Activating Compound

Activating Compound Comment Organism Structure
KHCO3 100 mM, slight activation Escherichia coli
NaOAc 100 mM, slight activation Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
N-terminal hexahistidine-tagged apo-H protein Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
octanoyl-CoA 0.5 mM, 67% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0102
-
 octanoyl-[acyl-carrier protein] pH 7.2, 37°C Escherichia coli
0.0132
-
 apo-H protein pH 7.2, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
 monomer, gel filtration Escherichia coli
29000
-
 trimer, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
octanoyl-[acyl-carrier protein] + apo-H protein Escherichia coli LipB reaction represents the first committed step in the biosynthesis of the lipoyl cofactor. apo-H protein is the lipoyl bearing subunit of the glycine cleavage system APO H-protein N6-(octanoyl)lysine + acyl-carrier protein
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
N-terminal hexahistidine-tagged apo-H protein Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.54
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
octanoyl-[acyl-carrier protein] + apo-H protein LipB reaction represents the first committed step in the biosynthesis of the lipoyl cofactor. apo-H protein is the lipoyl bearing subunit of the glycine cleavage system Escherichia coli APO H-protein N6-(octanoyl)lysine + acyl-carrier protein
-
 ?
octanoyl-[acyl-carrier protein] + apo-H protein apo-H protein is the lipoyl bearing subunit of the glycine cleavage system Escherichia coli apo-H protein N6-(octanoyl)lysine + acyl-carrier protein
-
 ?

Synonyms

Synonyms Comment Organism
LipB
-
 Escherichia coli
lipoyl(octanoyl)transferase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 octanoyl-[acyl-carrier protein] pH 7.2, 37°C Escherichia coli
0.2
-
 apo-H protein pH 7.2, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 65% of maximal activity, pH 9.0: about 40% of maximal activity Escherichia coli