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Literature summary for 2.3.1.180 extracted from
- A KAS-III heterodimer in lipstatin biosynthesis nondecarboxylatively condenses C8 and C14 fatty acyl-CoA substrates by a variable mechanism during the establishment of a C22 aliphatic skeleton (2019), J. Am. Chem. Soc., 141, 3993-4001 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces toxytricini |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E103A | the mutant of subunit LstA retains condensation activity | Streptomyces toxytricini |
| E154A | the mutant of subunit LstA retains condensation activity | Streptomyces toxytricini |
| E60A | inactive | Streptomyces toxytricini |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2 mM used in assay conditions | Streptomyces toxytricini |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration | Streptomyces toxytricini |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + a malonyl-[acyl-carrier protein] | Streptomyces toxytricini | - |
an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces toxytricini | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Streptomyces toxytricini |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E)-oct-2-enethioyl-CoA + (3S,5Z,8Z)-3-hydroxytetradeca-5,8-dienoyl-CoA | - |
Streptomyces toxytricini | (3S,5S,7Z,10Z)-2-hexyl-3,5-dihydroxyhexadeca-7,10-dienoic acid + ? | - |
? | |
| acetyl-CoA + a malonyl-[acyl-carrier protein] | - |
Streptomyces toxytricini | an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| octanoyl-CoA + (3S,5Z,8Z)-3-hydroxytetradeca-5,8-dienoyl-CoA | - |
Streptomyces toxytricini | (5S,7Z,10Z)-2-hexyl-5-hydroxy-3-oxohexadeca-7,10-dienoic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 40200 + 1 * 29300, subunits LstA and LstB | Streptomyces toxytricini |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-ketoacyl-acyl carrier protein synthase-III | - |
Streptomyces toxytricini |
| KAS-III | - |
Streptomyces toxytricini |
| LstA | subunit | Streptomyces toxytricini |
| LstAB | - |
Streptomyces toxytricini |
| LstB | subunit | Streptomyces toxytricini |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the LstAB protein plays a role in the biosynthesis of lipstatin | Streptomyces toxytricini |
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Release 2023.1 (January 2023)
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