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Literature summary for 2.3.1.180 extracted from

  • Kuo, J.; Khosla, C.
    The initiation ketosynthase (FabH) is the sole rate-limiting enzyme of the fatty acid synthase of Synechococcus sp. PCC 7002 (2014), Metab. Eng., 22, 53-59.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.180

Cloned(Commentary)

Cloned (Comment) Organism
gene fabH, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Synechococcus sp.

Protein Variants

Protein Variants Comment Organism
additional information the Synechococcus FabH is used for replacement of its counterpart in the reconstituted Escherichia coli fatty acid synthase system, the resulting complex is strongly limited in FabH activity. The introduction of the Escherichia coli FabH enzyme into the Synechococcus fatty acid synthase system virtually eliminates its dependence on the FabH subunit Synechococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + a malonyl-[acyl-carrier protein] Synechococcus sp.
-
 an acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Synechococcus sp. B1XPH5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and anion exchange chromatography, followed by ultrafiltration Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + a malonyl-[acyl-carrier protein]
-
 Synechococcus sp. an acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
additional information Product profile analysis of FabH from Synechococcus sp. strain PCC 7002, overview. t´The major products are lauric acid, myristic acid, palmitic acid, and stearic acid Synechococcus sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
FabH
-
 Synechococcus sp.
initiation ketosynthase
-
 Synechococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23 25 assay at room temperature Synechococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Synechococcus sp.

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Synechococcus sp.

General Information

General Information Comment Organism
metabolism the initiation ketosynthase FabH is the sole rate-limiting enzyme of the fatty acid synthesis of Synechococcus sp. PCC 700. Reconstitution of the fatty acid synthase system, FAS. FabH ketosynthase initiates product synthesis by condensing malonyl-ACP with acetyl-CoA to form acetoacetyl-ACP Synechococcus sp.