Cloned (Comment) | Organism |
---|---|
gene fabH, overexpression of His-tagged wild-type and mutant FabHs in Mycobacterium bovis strain BCG | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
mapping of the Thr45 phosphorylation site on the mtFabH crystal structure, overview | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
T45A | the mutant exhibits slightly decreased transacylation, malonyl-AcpM decarboxylation, and condensing activities compared to the wild-type protein | Mycobacterium tuberculosis |
T45D | the mutant exhibits markedly decreased transacylation, malonyl-AcpM decarboxylation, and condensing activities compared to the wild-type protein | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis H37Rv | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
additional information | Mycobacterium tuberculosis | FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNG3 | gene fabH | - |
Mycobacterium tuberculosis H37Rv | P9WNG3 | gene fabH | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | FabH is efficiently phosphorylated in vitro exclusively on Thr45 by several mycobacterial Ser/Thr protein kinases, particularly by PknF and PknA, as well as in vivo in mycobacteria, mass spectrometric analysis, overview. Mapping of the Thr45 phosphorylation site on the FabH crystal structure | Mycobacterium tuberculosis |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant FabHs from Mycobacterium bovis strain BCG by nickel affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP | Mycobacterium tuberculosis | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis H37Rv | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP | Mycobacterium tuberculosis H37Rv | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
additional information | FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview | Mycobacterium tuberculosis | ? | - |
? | |
additional information | FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-ACP synthase III | - |
Mycobacterium tuberculosis |
beta-ketoacyl-acyl carrier protein synthase III | - |
Mycobacterium tuberculosis |
FabH | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
condensation and decarboxylation assays at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
condensation assay at | Mycobacterium tuberculosis |
7.5 | - |
decarboxylation assay at | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP. FabH represents a potential regulatory key point of the mycolic acid pathway | Mycobacterium tuberculosis |