Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.180 extracted from

  • Veyron-Churlet, R.; Molle, V.; Taylor, R.C.; Brown, A.K.; Besra, G.S.; Zanella-Cleon, I.; Fuetterer, K.; Kremer, L.
    The Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III activity is inhibited by phosphorylation on a single threonine residue (2009), J. Biol. Chem., 284, 6414-6424.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene fabH, overexpression of His-tagged wild-type and mutant FabHs in Mycobacterium bovis strain BCG Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
mapping of the Thr45 phosphorylation site on the mtFabH crystal structure, overview Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
T45A the mutant exhibits slightly decreased transacylation, malonyl-AcpM decarboxylation, and condensing activities compared to the wild-type protein Mycobacterium tuberculosis
T45D the mutant exhibits markedly decreased transacylation, malonyl-AcpM decarboxylation, and condensing activities compared to the wild-type protein Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein] Mycobacterium tuberculosis FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2
-
?
acetyl-CoA + malonyl-[acyl-carrier protein] Mycobacterium tuberculosis H37Rv FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2
-
?
additional information Mycobacterium tuberculosis FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview ?
-
?
additional information Mycobacterium tuberculosis H37Rv FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNG3 gene fabH
-
Mycobacterium tuberculosis H37Rv P9WNG3 gene fabH
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein FabH is efficiently phosphorylated in vitro exclusively on Thr45 by several mycobacterial Ser/Thr protein kinases, particularly by PknF and PknA, as well as in vivo in mycobacteria, mass spectrometric analysis, overview. Mapping of the Thr45 phosphorylation site on the FabH crystal structure Mycobacterium tuberculosis

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant FabHs from Mycobacterium bovis strain BCG by nickel affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2
-
?
acetyl-CoA + malonyl-[acyl-carrier protein] FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP Mycobacterium tuberculosis 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2
-
?
acetyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis H37Rv 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2
-
?
acetyl-CoA + malonyl-[acyl-carrier protein] FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP Mycobacterium tuberculosis H37Rv 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2
-
?
additional information FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview Mycobacterium tuberculosis ?
-
?
additional information FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview Mycobacterium tuberculosis H37Rv ?
-
?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-ACP synthase III
-
Mycobacterium tuberculosis
beta-ketoacyl-acyl carrier protein synthase III
-
Mycobacterium tuberculosis
FabH
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
condensation and decarboxylation assays at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
condensation assay at Mycobacterium tuberculosis
7.5
-
decarboxylation assay at Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP. FabH represents a potential regulatory key point of the mycolic acid pathway Mycobacterium tuberculosis