Literature summary for 2.3.1.180 extracted from

Huynh, K.H.; Natarajan, S.; Song, N.H.; Ngo, P.T.; Ahn, Y.J.; Kim, J.G.; Lee, B.M.; Eo, Y.D.; Kang, L.W.
Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of beta-ketoacyl-ACP synthase III (FabH) from Xanthomonas oryzae pv. oryzae (2009), Acta Crystallogr. Sect. F, 65, 460-462.

Search for more literature for 2.3.1.180

Cloned(Commentary)

Cloned (Comment)	Organism
gene fabH or Xoo4209, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)	Xanthomonas oryzae pv. oryzae

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged KASIII, by sitting-drop vapour-diffusion method, 4 mg/ml proteinin 25 mM Tris-HCl, pH 7.5, 20% glycerol, 3 mM 2-mercaptoethanol, 1 M NaCl is mixed with precipitatnt solution containing 0.1 M HEPES, pH 7.2, 30% PEG 6000, 5% 2-methyl-2,4-pentanediol and 3% D-galactose, X-ray diffraction structure determination and analysis at 2.05 A resolution	Xanthomonas oryzae pv. oryzae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	x * 35000, recombinant His-tagged enzyme, SDS-PAGE	Xanthomonas oryzae pv. oryzae

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas oryzae pv. oryzae	-	gene fabH or Xoo4209	-
Xanthomonas oryzae pv. oryzae Xoo KACC10331	-	gene fabH or Xoo4209	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography	Xanthomonas oryzae pv. oryzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	structure-function relationship, overview	Xanthomonas oryzae pv. oryzae	?	-	?
additional information	structure-function relationship, overview	Xanthomonas oryzae pv. oryzae Xoo KACC10331	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 35000, recombinant His-tagged enzyme, SDS-PAGE	Xanthomonas oryzae pv. oryzae
More	structure-function relationship, overview	Xanthomonas oryzae pv. oryzae

Synonyms

Synonyms	Comment	Organism
beta-ketoacyl-ACP synthase III	-	Xanthomonas oryzae pv. oryzae
FabH	-	Xanthomonas oryzae pv. oryzae

General Information

General Information	Comment	Organism
physiological function	FabH is an important enzyme in the elongation steps of fatty-acid biosynthesis	Xanthomonas oryzae pv. oryzae

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Release 2023.1 (January 2023)