Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression as GST-fusion enzyme in Escherichia coli | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
thiolactomycin | uncompetitive, IC50 is 0.7 mM | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
malonyl-[acyl-carrier protein] | pH 7.6, 30°C | Pisum sativum | |
0.016 | - |
acetyl-CoA | pH 7.6, 30°C | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, about, DNA sequence calculation | Pisum sativum |
63000 | 75000 | gel filtration | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | Pisum sativum | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | Q9FSD6 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 147fold from leaves by ammonium sulfate fractionation, ion exchange chromatography, hydrophobic interaction chromatography on a butyl resin, and affinity chromatography, recombinant GST-fusion enzyme from Escherichia coli by glutathione affinity chromatography, GST is cleaved off by thrombin | Pisum sativum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | catalytic residues are Cys123, His323, and Asn353 | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pisum sativum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Pisum sativum | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
additional information | the enzyme shows also acetyl-CoA-[acyl-carrier-protein] transacylase activity | Pisum sativum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, about, DNA sequence calculation | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
ACP synthase III | - |
Pisum sativum |
beta-ketoacyl-acyl carrier protein synthase III | - |
Pisum sativum |
KAS III | - |
Pisum sativum |
short-chain condensing enzyme | - |
Pisum sativum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Pisum sativum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.7 | - |
uncompetitive, IC50 is 0.7 mM | Pisum sativum | thiolactomycin |