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Literature summary for 2.3.1.180 extracted from

  • Abbadi, A.; Brummel, M.; Spener, F.
    Knockout of the regulatory site of 3-ketoacyl-ACP synthase III enhances short- and medium-chain acyl-ACP synthesis (2000), Plant J., 24, 1-9.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Cuphea wrightii

Protein Variants

Protein Variants Comment Organism
A294S site-directed mutagenesis, the mutant enzyme shows reduced activity and reduced sensitivity to decanoyl-ACP inhibition compared to the wild-type Cuphea wrightii
A295P site-directed mutagenesis, the mutant enzyme shows reduced activity and reduced sensitivity to decanoyl-ACP inhibition compared to the wild-type Cuphea wrightii
N291D site-directed mutagenesis, the mutant enzyme shows reduced activity and reduced sensitivity to decanoyl-ACP inhibition compared to the wild-type Cuphea wrightii

Inhibitors

Inhibitors Comment Organism Structure
lauroyl-ACP feedback inhibition; feedback inhibition Cuphea wrightii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein] Cuphea wrightii regulation of the enzyme activity is involved in regulation of fatty acid synthesis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
?
acetyl-CoA + malonyl-[acyl-carrier protein] Cuphea wrightii regulation of the enzyme activity is involved in regulation of the fatty acid synthesis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
?

Organism

Organism UniProt Comment Textmining
Cuphea wrightii P49244 isozyme KASIIIA, chloroplast precursor; isozyme KASIIIa
-
Cuphea wrightii P49245 isozyme KASIIIB; isozyme KASIIIb
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Cuphea wrightii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of the recombinant wild-type or mutant N291D enzymes in seed extracts of Brassica napus and of Cuphea lanceolata Cuphea wrightii
additional information
-
activity of the recombinant wild-type or N291D mutant enzymes in seed extracts of Brassica napus and of Cuphea lanceolata Cuphea wrightii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein]
-
Cuphea wrightii acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
?
acetyl-CoA + malonyl-[acyl-carrier protein] regulation of the enzyme activity is involved in regulation of fatty acid synthesis Cuphea wrightii acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
?
acetyl-CoA + malonyl-[acyl-carrier protein] regulation of the enzyme activity is involved in regulation of the fatty acid synthesis Cuphea wrightii acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
?

Synonyms

Synonyms Comment Organism
3-ketoacyl-ACP synthase III
-
Cuphea wrightii
KASIII
-
Cuphea wrightii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cuphea wrightii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Cuphea wrightii