Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Cuphea wrightii |
Protein Variants | Comment | Organism |
---|---|---|
A294S | site-directed mutagenesis, the mutant enzyme shows reduced activity and reduced sensitivity to decanoyl-ACP inhibition compared to the wild-type | Cuphea wrightii |
A295P | site-directed mutagenesis, the mutant enzyme shows reduced activity and reduced sensitivity to decanoyl-ACP inhibition compared to the wild-type | Cuphea wrightii |
N291D | site-directed mutagenesis, the mutant enzyme shows reduced activity and reduced sensitivity to decanoyl-ACP inhibition compared to the wild-type | Cuphea wrightii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lauroyl-ACP | feedback inhibition; feedback inhibition | Cuphea wrightii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | Cuphea wrightii | regulation of the enzyme activity is involved in regulation of fatty acid synthesis | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | Cuphea wrightii | regulation of the enzyme activity is involved in regulation of the fatty acid synthesis | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cuphea wrightii | P49244 | isozyme KASIIIA, chloroplast precursor; isozyme KASIIIa | - |
Cuphea wrightii | P49245 | isozyme KASIIIB; isozyme KASIIIb | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Cuphea wrightii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of the recombinant wild-type or mutant N291D enzymes in seed extracts of Brassica napus and of Cuphea lanceolata | Cuphea wrightii |
additional information | - |
activity of the recombinant wild-type or N291D mutant enzymes in seed extracts of Brassica napus and of Cuphea lanceolata | Cuphea wrightii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Cuphea wrightii | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | regulation of the enzyme activity is involved in regulation of fatty acid synthesis | Cuphea wrightii | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | regulation of the enzyme activity is involved in regulation of the fatty acid synthesis | Cuphea wrightii | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketoacyl-ACP synthase III | - |
Cuphea wrightii |
KASIII | - |
Cuphea wrightii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cuphea wrightii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Cuphea wrightii |